PMID: 11339287May 8, 2001Paper

Major human plasma lipid classes determined by quantitative high-performance liquid chromatography, their variation and associations with phospholipid fatty acids

Journal of Chromatography. B, Biomedical Sciences and Applications
T Seppänen-LaaksoR Hiltunen

Abstract

An HPLC method with evaporative light-scattering detection (ELSD) was optimized and validated for the simultaneous quantitation of cholesteryl esters (CEs), triacylglycerols (TGs), free cholesterol (FC) and phosphatidylcholine (PC) in human plasma. The separation of CEs from TGs, the most variable plasma lipid class, was improved by speeding up the gradient steps and by increasing the re-equilibration time between runs. The calibrations were made at levels of 0.14-14 microg lipid/injection. The intra- and inter-day precision values of the method ranged between 1.9 and 4.5 and 2.3-7.2% (RSD, n=6), respectively, including determinations at two concentration levels. In comparison to other lipid classes, quantitation of PC proved to be equally repeatable despite its lowest detector response. The relative recoveries varied from 97.0 to 110.3%, showing good accuracy of the method. The methodological variation of the lipid classes covered 0.6-3.1% of their total variation in the study population (n=48). The CE/FC ratio showed an even closer relationship with phospholipid linoleic acid (18:2n-6; r=0.65, P<0.001) than with serum cholesterol levels, while eicosapentaenoic acid (20:5n-3) was significantly associated with PC (r=0.41, P<0.0...Continue Reading

References

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Jul 1, 1983·British Medical Bulletin·M A Crawford
Dec 13, 1996·Journal of Chromatography. B, Biomedical Applications·T Seppänen-LaaksoJ Viikari

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Citations

Aug 13, 2010·Lipids·Romain ColasCatherine Calzada
Jan 10, 2013·Journal of Agricultural and Food Chemistry·Bertram FongCarmen Norris
Mar 9, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T Seppänen-LaaksoR Hiltunen
Sep 3, 2005·Biomedical Chromatography : BMC·Brianna L Peterson, Brian S Cummings
Jan 22, 2013·Biotechnology and Bioengineering·Spyridon KonstantinidisNigel Titchener-Hooker
Mar 16, 2011·Analytical Biochemistry·David G McLarenThomas P Roddy
May 27, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Yufei Hu, Zhujun Zhang
Jul 16, 2011·Journal of Proteomics·Piero Del BoccioAndrea Urbani
May 10, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Eisaku HokazonoSusumu Osawa
Jul 28, 2016·Journal of the American College of Nutrition·Juristo Fonolla-JoyaManuel Muñoz-Torres
Aug 25, 2016·Journal of Chromatography. a·Nolwenn PrachePierre Chaminade
Oct 18, 2011·Journal of Separation Science·Christian HellmuthBerthold Koletzko
Nov 19, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Bruce C FrierDavid C Wright
Sep 26, 2012·Clinical Chemistry and Laboratory Medicine : CCLM·Nina FirlMichael Rychlik
Dec 19, 2018·Biomolecules·Spiro KhouryJustine Bertrand-Michel
Jan 29, 2021·BioMed Research International·Jialing LiuZhihe Zhao

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