PMID: 291049Aug 1, 1979Paper

Major intracellular cations and growth control: correspondence among magnesium content, protein synthesis, and the onset of DNA synthesis in BALB/c3T3 cells

Proceedings of the National Academy of Sciences of the United States of America
A H RubinH Sanui

Abstract

Omission of Ca(2+) from the medium of confluent BALB/c3T3 cells for a period of 17 hr causes a large decrease in the number of cells synthesizing DNA. This effect is reversed by raising the Mg(2+) concentration of the medium to 20 mM. However, if the [Mg(2+)] is greater than 20 mM ("ultra-high" Mg(2+)), there is again a decrease in the number of cells synthesizing DNA. The synthesis of protein has a similar dependence on Mg(2+) concentration in Ca(2+)-deficient medium, but it responds within 45 min of the shift in cation concentrations rather than the 10 hr that is required for the change in DNA synthesis to become apparent. Cells in the ultrahigh Mg(2+) concentrations that are at first inhibitory to protein synthesis later return to maximal protein synthesis. This delayed increase in protein synthesis is reflected in a delayed increase in DNA synthesis. Intracellular concentrations of Mg(2+) in Ca(2+)-deficient media increase in proportion to extracellular Mg(2+) concentrations. Cells in medium with 30 mM Mg(2+) have a high intracellular content of Mg(2+) at 3 hr but have decreased their intracellular content by 17 hr, a time at which protein synthesis has been restored to normal. Intracellular Na(+) and K(+) concentrations al...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·H Rubin
Aug 1, 1975·Journal of Cellular Physiology·H Rubin, T Koide
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A H RubinH Sanui
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D F Bowen-PopeA H Rubin
Aug 1, 1978·Journal of Cellular Physiology·G C Baxter, C P Stanners
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H Rubin, T Koide
Mar 15, 1977·Experimental Cell Research·M L Ledbetter, M Lubin
Jun 2, 1977·Nature·E RozengurtN M Wigglesworth
Sep 1, 1977·Journal of Cellular Physiology·L N Castor
Dec 12, 1973·Nature: New Biology·D W Fodge, H Rubin
Jan 1, 1974·Advances in Enzyme Regulation·A J Lostroh, M E Krahl
Feb 15, 1963·Proceedings of the National Academy of Sciences of the United States of America·M VOGT, R DULBECCO
Sep 1, 1964·Journal of Molecular Biology·M MESELSOND SCHLESSINGER

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Citations

Jul 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A Knedler, R G Ham
Aug 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Harry Rubin
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H Rubin
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H RubinH Sanui
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M Terasaki, H Rubin
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S B Horowitz, L J Tluczek
Oct 1, 1982·The Journal of Cell Biology·T J RinkT Pozzan
Jan 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Charles Vidair, Harry Rubin
Feb 17, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Harry Rubin
Sep 1, 1981·Journal of Cellular Physiology·C Vidair, H Rubin
Jan 1, 1986·Journal of the American College of Nutrition·P Fort, F Lifshitz
Dec 30, 2014·Physiological Reviews·Jeroen H F de BaaijRené J M Bindels
Jun 1, 1981·The Journal of Investigative Dermatology·H PatelL A Diaz

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