Major outer membrane proteins and proteolytic processing of RgpA and Kgp of Porphyromonas gingivalis W50

The Biochemical Journal
Paul D VeithE C Reynolds

Abstract

Porphyromonas gingivalis is an anaerobic, asaccharolytic Gram-negative rod associated with chronic periodontitis. We have undertaken a proteomic study of the outer membrane of P. gingivalis strain W50 using two-dimensional gel electrophoresis and peptide mass fingerprinting. Proteins were identified by reference to the pre-release genomic sequence of P. gingivalis available from The Institute for Genomic Research. Out of 39 proteins identified, five were TonB-linked outer membrane receptors, ten others were putative integral outer membrane proteins and four were putative lipoproteins. Pyroglutamate was found to be the N-terminal residue of seven of the proteins, and was predicted to be the N-terminal residue of 13 additional proteins. The RgpA, Kgp and HagA polyproteins were identified as fully processed domains in outer membranes prepared in the presence of proteinase inhibitors. Several domains were found to be C-terminally truncated 16-57 residues upstream from the N-terminus of the following domain, at a residue penultimate to a lysine. This pattern of C-terminal processing was not detected in a W50 strain isogenic mutant lacking the lysine-specific proteinase Kgp. Construction of another W50 isogenic mutant lacking the arg...Continue Reading

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Citations

Sep 14, 2011·The Journal of Biological Chemistry·Gerald PoschChristina Schäffer
May 18, 2012·The Journal of Biological Chemistry·Michelle D GlewEric C Reynolds
Nov 9, 2012·Molecular Oral Microbiology·D OsbourneH M Fletcher
Dec 22, 2010·Antimicrobial Agents and Chemotherapy·Elena C Y TohEric C Reynolds
Mar 7, 2007·Infection and Immunity·Rishi D PathiranaEric C Reynolds
Feb 26, 2005·Infection and Immunity·Elaine VanterpoolHansel M Fletcher
Aug 7, 2004·Journal of Clinical Microbiology·Mangala A NadkarniNeil Hunter
Sep 5, 2012·Biological Chemistry·Maryta SztukowskaJan Potempa
May 7, 2009·Future Microbiology·Rebecca E FitzpatrickRobert N Pike
Aug 12, 2014·Journal of Proteomics·Michelle D GlewEric C Reynolds
Dec 18, 2003·Oral Microbiology and Immunology·S G DashperE C Reynolds
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
Nov 1, 2008·Journal of Periodontal Research·F YoshimuraS Kawaminami
Dec 19, 2009·Periodontology 2000·Rishi D PathiranaEric C Reynolds
Oct 13, 2015·Microbial Pathogenesis·Mikio Shoji, Koji Nakayama
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Jun 13, 2015·Mediators of Inflammation·Sim K SinghraoStJohn Crean
Mar 23, 2017·Scientific Reports·Rebecca L J MelcherBruno M Moerschbacher
Mar 1, 2011·European Journal of Microbiology & Immunology·N Li, C A Collyer
Sep 15, 2012·Nihon saikingaku zasshi. Japanese journal of bacteriology·Mikio Shoji

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