Major role of pKpQIL-like plasmids in the early dissemination of KPC-type carbapenemases in the UK

The Journal of Antimicrobial Chemotherapy
M DoumithN Woodford

Abstract

Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae were first seen in the UK in 2003 and have been increasingly reported since 2010, largely owing to an ongoing outbreak in North-West England. We examined the role of clonal spread and plasmid transmission in their emergence. Isolates comprised KPC-positive K. pneumoniae ( n  =   33), Escherichia coli ( n  =   7) and Enterobacter spp. ( n  =   4) referred to the national reference laboratory between 2008 and 2010 from 17 UK centres, including three in North-West England. Isolates were typed by MLST. Plasmids were transferred by electroporation and characterized by PCR or sequencing. PCR screening assays were developed to distinguish plasmid pKpQIL variants. The K. pneumoniae isolates included 10 STs, of which three belonged to clonal group (CG) 258. CG258 ( n  =   19) isolates were detected in 13 centres but accounted for only 7/19 (36.8%) of those from North-West England. Most KPC-producers (37/44, 84.1%), including 16/19 CG258 K. pneumoniae , carried bla KPC on IncFII K2 plasmids. Sequencing of a subset of these plasmids ( n  =   11) revealed similarities with published pKpQIL. One variant, pKpQIL-UK [identified in K. pneumoniae CG258 ( n  =   5) and ST468 ...Continue Reading

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Citations

May 8, 2019·Antimicrobial Agents and Chemotherapy·Maria KarlssonAlison Laufer Halpin
Mar 7, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Catherine LuddenSharon J Peacock
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May 10, 2020·The Journal of Antimicrobial Chemotherapy·Agnès B JoussetThierry Naas
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Apr 17, 2019·The Journal of Antimicrobial Chemotherapy·C M RooneyC H Chilton
Aug 31, 2019·Future Microbiology·Serena SimoniMarina Mingoia
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Aug 4, 2019·Scientific Reports·Christian BrandtOliwia Makarewicz
Nov 21, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Jorge ReyesGabriel Trueba
Mar 16, 2021·International Journal of Antimicrobial Agents·Shazad MushtaqDavid M Livermore
Aug 3, 2021·Antimicrobial Agents and Chemotherapy·Alessandra CarattoliGiammarco Raponi

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