Major T cell progenitor activity in bone marrow-derived spleen colonies

The Journal of Experimental Medicine
Christophe LancrinSophie Ezine

Abstract

Common lymphoid progenitors (CLP) are generated in adult bone marrow (BM), but the intermediate steps leading to T cell commitment are unknown, and so is the site at which this commitment occurs. Here, we show that colonies arising in the spleen 12 days after BM injection harbor T cell precursors that are undetectable in BM. These precursors did not generate myeloid cells in vivo but repopulated the thymus and the peripheral T cell compartment much faster than did CLP. Two lineage negative (Lin(-)) subpopulations were distinguished, namely CD44(+) Thy1(-) cells still capable of natural killer generation and transient low-level B cell generation, and T cell-restricted CD44(-) Thy1(+) cells. At a molecular level, frequency of CD3epsilon and preTalpha mRNA was very different in each subset. Furthermore, only the CD44(-) Thy1(+) subset have initiated rearrangements in the T cell receptor beta locus. Thus, this study identifies extramedullary T cell progenitors and will allow easy approach to T cell commitment studies.

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Citations

Feb 24, 2006·Nature Reviews. Immunology·Avinash Bhandoola, Arivazhagan Sambandam
Jun 28, 2011·Nature Reviews. Immunology·Paul E Love, Avinash Bhandoola
Jan 31, 2007·The Journal of Experimental Medicine·Valerie BesseyriasFreddy Radtke
Oct 18, 2008·Seminars in Immunopathology·Daniel A ZlotoffAvinash Bhandoola
Jul 16, 2010·Seminars in Immunology·Dil Afroz SultanaAvinash Bhandoola
Aug 25, 2009·European Journal of Immunology·Arkadiusz MiazekPawel Kisielow
Feb 13, 2007·Immunological Reviews·Benedita Rocha
Jan 22, 2011·Annals of the New York Academy of Sciences·Daniel A Zlotoff, Avinash Bhandoola
Sep 21, 2013·Blood Reviews·Christen L Ebens, Ivan Maillard
Dec 13, 2003·Immunity·Ivan MaillardWarren S Pear
Nov 5, 2002·Nature Reviews. Immunology·Rod Ceredig, Ton Rolink
Jan 6, 2004·Nature Reviews. Immunology·Juan Carlos Zúñiga-Pflücker
May 31, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vanessa ZepponiSophie Ezine
Nov 25, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Avinash BhandoolaBenjamin Schwarz
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laetitia GautreauSophie Ezine
Feb 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marie-Laure ArcangeliSophie Ezine
Feb 25, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kyoko MasudaHiroshi Kawamoto
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benjamin A SchwarzAvinash Bhandoola

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Methods Mentioned

BETA
Transgenic
flow cytometry
Assay
PCR
fluorescence-activated cell sorting

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