Make them blink: probes for super-resolution microscopy

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Jan VogelsangPhilip Tinnefeld

Abstract

In recent years, a number of approaches have emerged that enable far-field fluorescence imaging beyond the diffraction limit of light, namely super-resolution microscopy. These techniques are beginning to profoundly alter our abilities to look at biological structures and dynamics and are bound to spread into conventional biological laboratories. Nowadays these approaches can be divided into two categories, one based on targeted switching and readout, and the other based on stochastic switching and readout of the fluorescence information. The main prerequisite for a successful implementation of both categories is the ability to prepare the fluorescent emitters in two distinct states, a bright and a dark state. Herein, we provide an overview of recent developments in super-resolution microscopy techniques and outline the special requirements for the fluorescent probes used. In combination with the advances in understanding the photophysics and photochemistry of single fluorophores, we demonstrate how essentially any single-molecule compatible fluorophore can be used for super-resolution microscopy. We present examples for super-resolution microscopy with standard organic fluorophores, discuss factors that influence resolution an...Continue Reading

References

Dec 4, 1998·Science·H P LuX S Xie
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Edman, R Rigler
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·T A KlarS W Hell
Apr 20, 2002·Biophysical Journal·Russell E ThompsonWatt W Webb
Aug 3, 2002·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Rainer HeintzmannChristoph Cremer
Sep 14, 2002·Science·George H Patterson, Jennifer Lippincott-Schwartz
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ryoko AndoAtsushi Miyawaki
Dec 14, 2002·Angewandte Chemie·Hannes NeuweilerMarkus Sauer
Dec 20, 2002·Nature·Masahiro IrieTsuyoshi Kawai
Dec 24, 2002·Nature Structural Biology·Sean A McKinneyTaekjip Ha
Nov 5, 2003·Nature Biotechnology·Elizabeth A Jares-Erijman, Thomas M Jovin
Dec 4, 2003·Journal of the American Chemical Society·Jeremy R BabendureRoger Y Tsien
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Matthew P GordonPaul R Selvin
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xiaohui QuNorbert F Scherer
Oct 7, 2004·Current Opinion in Neurobiology·Stefan W HellStefan Jakobs
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jörg WiedenmannG Ulrich Nienhaus
Dec 1, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M Consuelo JiménezIgnacio Vayá
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ophir FlomenbomJoseph Klafter
Feb 26, 2005·EMBO Reports·Hidekazu TsutsuiAtsushi Miyawaki
Mar 18, 2005·Journal of the American Chemical Society·Mike HeilemannPhilip Tinnefeld
Mar 24, 2005·Physical Review Letters·Mark BatesXiaowei Zhuang
May 11, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Thomas HeinleinMarkus Sauer
Jun 23, 2005·Journal of the American Chemical Society·Linyong ZhuAlexander D Q Li
Sep 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mats G L Gustafsson
Sep 17, 2005·Nature Reviews. Molecular Cell Biology·Konstantin A LukyanovVladislav V Verkhusha
Oct 4, 2005·Physical Review Letters·Jacob P HoogenboomMaría F García-Parajó
Nov 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Michael HofmannStefan W Hell
Mar 3, 2006·The Journal of Physical Chemistry. a·Christian EggelingClaus A M Seidel
Mar 9, 2006·Nano Letters·Chittanon BuranachaiTaekjip Ha
Mar 17, 2006·Nature·Paul W K Rothemund
Mar 22, 2006·Nature Biotechnology·Nadya G GurskayaKonstantin A Lukyanov
Apr 25, 2006·Physical Chemistry Chemical Physics : PCCP·Erwen MeiRobin M Hochstrasser
Jun 30, 2006·Applied Optics·Rainer Heintzmann, Pier A Benedetti
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 10, 2006·Nature Methods·Katrin I WilligStefan W Hell
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
Oct 3, 2006·Nature Methods·Ivan RasnikTaekjip Ha
Dec 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alexey Sharonov, Robin M Hochstrasser
Jan 19, 2007·The Journal of Physical Chemistry. a·Jerker WidengrenClaus A M Seidel
Jan 31, 2007·Microscopy Research and Technique·Miriam A SchwentkerStefan W Hell
Mar 23, 2007·Journal of the American Chemical Society·Xiangxu KongShimon Weiss
May 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tom VoschRobert M Dickson
May 26, 2007·Science·Stefan W Hell
May 29, 2007·The Journal of Physical Chemistry. B·John N CliffordEdwin K L Yeow
Jul 21, 2007·Angewandte Chemie·J FöllingS W Hell
Jul 31, 2007·Biophysical Journal·Alexander EgnerStefan W Hell

❮ Previous
Next ❯

Citations

Jun 20, 2012·Journal of Fluorescence·Stefan V Stoianov, Hans D Robinson
Dec 25, 2010·Journal of the American Chemical Society·Ibrahim YildizFrançisco M Raymo
Aug 31, 2011·Journal of the American Chemical Society·Kiyohiko KawaiTetsuro Majima
Sep 22, 2012·The Journal of Physical Chemistry. a·Subramani SwaminathanFrançisco M Raymo
May 20, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Erhan DenizFrançisco M Raymo
Aug 16, 2011·Nano Letters·Ingmar SchoenViola Vogel
Oct 30, 2012·Nature Methods·Joshua C VaughanXiaowei Zhuang
Jul 2, 2011·Nature Protocols·Sebastian van de LindeMarkus Sauer
Feb 12, 2011·Physical Chemistry Chemical Physics : PCCP·Thorben CordesPhilip Tinnefeld
May 24, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Britta SeefeldtJochen Mattay
Jun 23, 2012·Physical Chemistry Chemical Physics : PCCP·Erhan DenizFrançisco M Raymo
Apr 24, 2012·Nucleic Acids Research·Andreas GietlPhilip Tinnefeld
Dec 4, 2012·International Journal of Molecular Sciences·Erin A RileyPhilip J Reid
Mar 22, 2014·Soft Matter·Stefan Howorka, Jan Hesse
May 17, 2014·Nature Protocols·Jürgen J SchmiedPhilip Tinnefeld
Mar 13, 2012·Annual Review of Physical Chemistry·Taekjip Ha, Philip Tinnefeld
Mar 13, 2012·Annual Review of Physical Chemistry·Sebastian van de LindeMarkus Sauer
Nov 6, 2014·Nature Communications·Phil HolzmeisterPhilip Tinnefeld
Aug 8, 2014·Chemical Society Reviews·Lanlan SunKasper Moth-Poulsen
Aug 19, 2015·The Journal of Physical Chemistry Letters·Jasper H M van der VeldeThorben Cordes
Aug 10, 2013·The Journal of Physical Chemistry Letters·Jianmin XuShimon Weiss
Aug 24, 2012·The Journal of Physical Chemistry. B·Erin A RileyPhilip J Reid
Oct 2, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexander GustDina Grohmann
Aug 16, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beth L HaasJulie S Biteen
Jan 17, 2016·Current Opinion in Biotechnology·Julia MollePhilip Tinnefeld
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Enrico KlotzschElena E Pohl
Nov 29, 2011·Current Opinion in Chemical Biology·Suliana ManleyNicolas Olivier
Nov 22, 2011·Current Opinion in Chemical Biology·Cristina Flors, William C Earnshaw
May 4, 2011·Current Opinion in Chemical Biology·David KlenermanSimon J Davis
Oct 30, 2010·Current Opinion in Biotechnology·Marlon J Hinner, Kai Johnsson
Jun 2, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Antoine Le GallNathalie Westbrook
Dec 14, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ingo H SteinPhilip Tinnefeld
May 31, 2012·Chemistry : a European Journal·Janet CusidoFrançisco M Raymo
May 23, 2013·Photochemistry and Photobiology·Marco PetriellaMariano L Bossi
Sep 17, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Monika A Ciuba, Marcia Levitus
Jun 15, 2011·Chemistry : a European Journal·Wu SuGlenn A Burley
Oct 31, 2012·Chemistry : a European Journal·Erhan DenizSalvatore Sortino
Nov 25, 2011·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Ralf JungmannFriedrich C Simmel
Mar 16, 2011·Angewandte Chemie·Michael SchweringDirk-Peter Herten
Nov 15, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Thorge HolmMarkus Sauer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.