Making CENs of mammalian artificial chromosomes

Molecular Genetics and Metabolism
P E Warburton

Abstract

Mammalian artificial chromosomes (MACs) hold the promise of providing autonomous vectors for gene therapy in dividing cells. They would not require insertion into the genome and could include sufficient genomic sequences that surround the therapeutic gene to ensure proper tissue-specific and temporal regulation. Several groups have reported successful formation of MACs in human cells using transfection strategies that included alpha satellite DNA, the primary DNA found at normal human centromeres. These results, although extremely encouraging, have limitations such as unpredictable chromosome formation and success thus far in only one transformed human cell line. Examination of other cells where alpha satellite DNA has integrated into ectopic chromosomal locations, as well as naturally occurring dicentric and neocentromere-containing cell lines, suggests that alpha satellite DNA may not be necessary or sufficient for centromere formation. Overall, these results suggest that epigenetic modifications of centromeric DNA are required for efficient centromere formation. Models for this centromere-specific epigenetic modification include a specialized chromatin structure and differential replication timing of centromeric DNA. Thus, f...Continue Reading

References

Mar 27, 2001·EMBO Reports·G FloridiaC Tyler-Smith
Dec 12, 2012·Molecular and Cellular Biology·Karen E HaydenH F Willard

Citations

Sep 25, 1991·Nucleic Acids Research·S B Haase, M P Calos
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C FarrH Cooke
Dec 1, 1990·Trends in Genetics : TIG·H F Willard
Dec 1, 1990·Molecular and Cellular Biology·K G Ten HagenS N Cohen
Sep 1, 1995·Trends in Genetics : TIG·W Brown, C Tyler-Smith
Dec 8, 1995·Science·A F PlutaW C Earnshaw
Aug 1, 1994·Human Molecular Genetics·K E BrownW R Brown
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·J P HanishT de Lange
Oct 11, 1996·Science·Y Li, R Benezra
Apr 1, 1997·Trends in Genetics : TIG·D Kipling, P E Warburton
Dec 18, 1997·American Journal of Human Genetics·K H Choo
Jan 13, 1998·Nature Genetics·K H Choo
Jan 13, 1998·Nature Genetics·B C WilliamsG H Karpen
Jan 20, 1998·Trends in Genetics : TIG·G H Karpen, R C Allshire
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·P KalitsisK H Choo
Jun 4, 1998·Current Opinion in Genetics & Development·H F Willard
Jun 5, 1998·Trends in Genetics : TIG·A K Csink, S Henikoff
Aug 5, 1998·Current Opinion in Genetics & Development·J M Vos
Sep 15, 1998·Human Molecular Genetics·B Grimes, H Cooke
Feb 5, 1999·Human Molecular Genetics·E E Eichler
Feb 5, 1999·Chromosoma·M KapoorG S May

Related Concepts

Shuttle Vectors
Transfection
Genome
Chromosomes, Artificial, Human
Centromere
Chromosomes, Artificial, Mammalian
DNA Replication Timing
Study of Epigenetics
Human Cell Line
Genomics

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