Malaria DNA vaccine gp96NTD-CSP elicits both CSP-specific antibody and CD8(+) T cell response

Parasitology Research
Zhangping TanWenyue Xu

Abstract

It is ideal for the pre-erythrocytic stage subunit vaccine to induce both CSP-specific antibody and CD8(+) T cell response. Here, we designed a novel malaria DNA vaccine gp96NTD-CSP, which was constructed by fusing the full-length of CSP with the N-terminal domain of gp96 that deleted the endoplasmic reticulum-localized motif KDEL, and investigated its protective efficacy. We found that the fusion protein gp96NTD-CSP was mainly distributed on the surface of eukaryotic cells after transfection and could be sensed as a "danger signal" by the host immune system. Interestingly, both liver parasite burden and parasitemia in mice immunized with gp96NTD-CSP were significantly lower than those in the mice immunized either with gp96NTD, CSP, or gp96NTD-SYVPSAEQI, which was constructed by fusing the CSP-specific CD8(+) T cell epitope with the N-terminal domain of gp96 deleted with KDEL. Consistently, both the level of CSP-specific antibody and the frequency of IFN-γ secreted-CSP-specific CD8(+) T cells were much higher in mice immunized with gp96NTD-CSP than those in the mice immunized either with gp96NTD, CSP, or gp96NTD-SYVPSAEQI. Our results suggest that the malaria DNA vaccine gp96NTD-CSP could induce both humoral and cellular immune...Continue Reading

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Citations

Dec 23, 2016·Journal of Medical Microbiology·Marcelle Moura SilveiraÂngela Nunes Moreira
Dec 21, 2018·Molecular Medicine Reports·Bo ChenGuizhen Wang
Aug 22, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tayla M OlsenSean C Murphy
Feb 28, 2019·Frontiers in Veterinary Science·Valeria A SanderMarina Clemente
Feb 9, 2021·Frontiers in Bioengineering and Biotechnology·Mariana G CoriglianoMarina Clemente

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