Malaria Panel Assay versus PCR: detection of naturally infected Anopheles melas in a coastal village of Equatorial Guinea

Malaria Journal
Marta MorenoA Benito

Abstract

A study was carried out in a village of the mainland region of Equatorial Guinea in order to ascertain a) which members of Anopheles gambiae complex could be involved in malaria transmission and b) the rate of infectivity for Anopheles melas comparing two different methods, a PCR able to detect sporozoite-DNA and an immunochromatographic assay MPR (Malaria Rapid Dipstick Panel Assay). Mosquitoes were sampled at night by indoor captures in two houses of a coastal village in Equatorial Guinea (Ayantang). Collected mosquitoes were identified as An. gambiae s.l. These were individually dried into silica-gel. The head-thorax of the An. gambiae s.l. mosquitoes were analysed by PCR to verify that the species was of the gambiae complex. Individual head-thorax and pools (5 pools) of homogenized mosquitoes employed in Malaria Rapid Panel assay (MRP assay) were lysed and DNA was extracted. PCR was designed from the 753 base pair insert of pBRKl-14 and DNA was amplified. The relationship between dipstick and PCR to detect Plasmodium falciparum sporozoites was measured in terms of sensitivity, specificity and test association (Cohen's kappa value). Two hundred and sixty-four An. gambiae s.l. females were studied (214 individually and five p...Continue Reading

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Citations

Sep 2, 2011·Malaria Journal·Mallika ImwongNicholas J White
Apr 2, 2008·Biological & Pharmaceutical Bulletin·Akter TanginHye-Sook Kim
Dec 10, 2009·The American Journal of Tropical Medicine and Hygiene·Mariangela BonizzoniGuiyun Yan
Dec 6, 2008·The Journal of Veterinary Medical Science·Hiroko EjiriMasayoshi Yukawa
Mar 25, 2010·The Korean Journal of Parasitology·Sung Suck OhE Hyun Shin
Oct 1, 2015·Bulletin de la Société de pathologie exotique·P Ntonga AkonoL G Lehman
Jan 1, 2013·ISRN Parasitology·Doris A RoseroMargarita M Correa

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Methods Mentioned

BETA
dissection
enzyme-linked immunosorvent assay
PCR
electrophoresis
ELISA

Software Mentioned

MultiAnalyst

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