Malaria vectors of Timor-Leste.

Malaria Journal
R D CooperN W Beebe

Abstract

The island of Timor lies at the south-eastern edge of Indonesia on the boundary of the Oriental and Australian faunal regions. The country of Timor-Leste, which occupies the eastern part of the island, is malarious, but anopheline faunal surveys and malaria vector incrimination date back to the 1960 s. Over the last decade the malaria vectors of south-east Asia and the south-west Pacific have been intensely studied using molecular techniques that can confirm identification within complexes of isomorphic species. The aim of this study is to accurately identify the Anopheles fauna of Timor-Leste using these techniques. The survey was carried out over the period February to June 2001. Standard entomological techniques--human landing collections, larval collections and CO2 baited light traps--were used to collect anophelines from the main geographical regions: coastal plains, inland plains, and highlands. Specimens were processed for identification by morphology and genotyped for the ribosomal DNA ITS2 by restriction analysis and/or DNA sequencing. Phylogenetic relationship of Anopheles sundaicus and Anopheles subpictus individuals was also assessed using DNA sequences from the ITS2 and mitochondrial cytochrome-b. All specimens, ot...Continue Reading

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Citations

Nov 15, 2012·BMC Genomics·Albin FontaineLionel Almeras
Apr 30, 2014·Journal of the American Mosquito Control Association·Esther M Anderson, Jennifer A Davis
Sep 11, 2014·Journal of Insect Science·Pavillupillai J JudeSinnathamby N Surendran
Apr 19, 2015·Malaria Journal·Manel A M G YapabandaraAnanda R Wickremasinghe
Jun 7, 2018·Journal of Medical Entomology·Jalil NejatiAlireza Sanei-Dehkordi
Aug 29, 2017·Interdisciplinary Perspectives on Infectious Diseases·O R PinontoanI B P Manuaba

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Datasets Mentioned

BETA
AF543862

Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

ClustalW
PhyML

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