Malassezia sympodialis stimulation differently affects gene expression in dendritic cells from atopic dermatitis patients and healthy individuals

Acta Dermato-venereologica
Susanne GabrielssonA Scheynius

Abstract

It is known that 28-84% of patients with atopic dermatitis exhibit IgE and/or T-cell reactivity to the opportunistic yeast Malassezia sympodialis, which can be taken up by immature monocyte-derived dendritic cells (MDDCs), resulting in MDDC maturation. The aim of this study was to investigate whether MDDCs from patients with atopic dermatitis respond differently to M. sympodialis compared to MDDCs from healthy individuals. Immature MDDCs were stimulated with M. sympodialis and the gene expression profiles were analysed with cDNA arrays containing 406 genes. Our results show that M. sympodialis differently affected MDDCs from patients with atopic dermatitis, and more so in severely ill patients, compared with healthy individuals. Six genes were more than fivefold up-regulated in MDDCs from more than one patient with atopic dermatitis, coding for CD54, CD83, IL-8, monocyte-derived chemokine (MDC), BTG1 and IL-1R antagonist. In healthy individuals this was true only for BTG1. Up-regulations of IL-8 and MDC were confirmed at the protein level. Our findings might reflect an increased trafficking and stimulatory capacity in MDDCs from the patients, which is likely to result in a stronger inflammatory response to M. sympodialis.

Citations

Jun 17, 2006·The Journal of Investigative Dermatology·Barbra Fischer CasagrandePeter Schmid-Grendelmeier
Nov 22, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A RoesslerG Jahreis
Mar 6, 2012·The Journal of Allergy and Clinical Immunology·Natalija Novak
May 19, 2006·FEMS Immunology and Medical Microbiology·Helen Ruth Ashbee
Mar 26, 2019·Mycoses·Danuta Nowicka, Urszula Nawrot
Apr 13, 2010·Autoimmunity·Liam O'MahonyCezmi A Akdis

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