MALAT1: A regulator of inflammatory cytokines in diabetic complications

Endocrinology, Diabetes & Metabolism
Andrew Devon GordonSubrata Chakrabarti

Abstract

In this study, we examined the role of MALAT1, a highly conserved nuclear long non-coding RNA molecule, in chronic diabetic complications affecting the heart and kidneys using both in vitro and in vivo models: human endothelial cell culture and a Malat1 knockout mice model. Findings from our in vitro experiments demonstrated that MALAT1 was predominantly localized to nuclear speckles in endothelial cells and MALAT1 expression was significantly increased following incubation with high glucose in association with increased expression of inflammatory cytokines. As for our in vivo experiments, we used Malat1 knockout mice and wild-type controls with or without streptozotocin-induced diabetes over 2 months of follow-up, where all of our diabetic animals showed hyperglycaemia and polyuria. Examination of cardiac and renal tissues demonstrated altered MALAT1 RNA expression in wild-type diabetic animals. Such changes were associated with augmented production of downstream inflammatory molecules at the mRNA and protein levels. Diabetes-induced elevations of inflammatory markers were significantly decreased in Malat1 knockout diabetic animals. In addition to transcript and protein analyses, we examined functional changes in the heart and...Continue Reading

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Citations

Jul 25, 2019·Journal of Clinical Medicine·Saumik BiswasSubrata Chakrabarti
May 1, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Koen E GroenewegRoel Bijkerk
May 28, 2020·American Journal of Physiology. Cell Physiology·Erfan Aref-EshghiSubrata Chakrabarti
Jan 11, 2020·Frontiers in Endocrinology·Saumik BiswasSubrata Chakrabarti
Apr 20, 2018·Frontiers in Cardiovascular Medicine·Saumik BiswasSubrata Chakrabarti
Jan 7, 2019·Diabetologia·Anu A ThomasSubrata Chakrabarti
Oct 1, 2021·International Journal of Cardiology·Joakim SandstedtVictoria Rotter Sopasakis

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Methods Mentioned

BETA
histone acetylation
protein assay
ELISA
reverse

Software Mentioned

BLAST

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