MALAT1-miR663a negative feedback loop in colon cancer cell functions through direct miRNA-lncRNA binding

Cell Death & Disease
Wei TianDajun Deng

Abstract

The lncRNA MALAT1 has multiple biological functions, including influencing RNA processing, miRNA sponging, and cancer development. It is acknowledged that miR663a and its targets are inflammation-related genes frequently deregulated in many cancers. The associations between MALAT1 and miR663a and their target genes remain unknown. In this study, it was found that in colon cancer (CC) cells, MALAT1 and miR663a were reciprocally repressed in cDNA array screening and qRT-PCR analysis. However, MALAT1 was significantly upregulated in CC tissues, and miR663a was significantly downregulated relative to the corresponding surgical margin (SM) tissues. An inverse relationship between MALAT1 and miR663a expression was detected among CC tissue samples (n = 172, r = -0.333, p < 0.0001). The RNA-pulldown results showed MALAT1 lncRNA-miR663a binding. The results of luciferase-reporter analysis further revealed that the MALAT1 7038-7059 nt fragment was the miR663a seed sequence. Both miR663a knockdown and MALAT1 activation alone significantly upregulated the expression levels of miR663a targets, including TGFB1, PIK3CD, P53, P21, and JUND, in the CC cell lines HCT116 and SW480. A positive relationship was also observed between the expression ...Continue Reading

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Citations

Feb 1, 2020·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Guo-Min LiuYun-Gang Luo
Oct 27, 2018·International Journal of Oncology·Xiaotian ZhangYimin Chai
Jan 29, 2020·World Journal of Gastroenterology : WJG·Wan-Ru MaDa-Jun Deng
Mar 17, 2020·Cancer Biomarkers : Section a of Disease Markers·Rui Liang, Youjia Tang
Apr 28, 2020·Cancer Biology & Therapy·Haixia LiNie Tian
Aug 23, 2019·Molecular and Cellular Biochemistry·Wei XiongYi Yang
Nov 29, 2020·Molecular Cancer·Sian Chen, Xian Shen
Sep 22, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Fen-Rong ChenLei Dong
Jun 19, 2021·Biochemical Pharmacology·Yaaqub Abiodun UthmanMurtala Bello Abubakar
Jul 7, 2020·Anti-cancer Agents in Medicinal Chemistry·Palmiro PoltronieriGiovanna Giovinazzo

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Datasets Mentioned

BETA
GSE24551

Methods Mentioned

BETA
transfection
pull-down
PCR
reverse transcription-PCR
pulldown
pull down
Assay

Software Mentioned

IncuCyte
SPSS
IncuCyte ZOOM

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