PMID: 6990969May 13, 1980Paper

Malate dehydrogenase from thermophilic and mesophilic bacteria. Molecular size, subunit structure, amino acid composition, immunochemical homology, and catalytic activity

Biochemistry
T K SundaramA E Wilkinson

Abstract

Malate dehydrogenases isolated from a number of mesophilic, moderately thermophilic, and extremely thermophilic bacteria yield upon denaturation subunits of molecular weight 32 000--36 000. Determination of their native molecular weights shows that some of the enzymes are dimeric and others are tetrameric; the two types are distributed in each of the three classes of bacteria. The amino acid compositions of the enzymes show no consistent trend that can be related to the progression of thermostability from the mesophile through the moderate thermophile to the extreme thermophile species. The tetrameric enzyme species all exhibit a high level of structural homology as judged by the criterion of immunological cross-reaction. Little cross-reaction occurs, however, between the tetramers and the dimers. The dimeric enzyme from the extreme thermophile, Thermus aquaticus, cross-reacts only weakly, if at all, even with dimeric malate dehydrogenases. The catalytic activities of the malate dehydrogenases vary over a wide range. Potassium chloride, organic solvents such as acetone, and the protein denaturants urea and guanidine hydrochloride activate a number of the malate dehydrogenases under the assay conditions employed. The diversity a...Continue Reading

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