MALDI Imaging Analysis of Neuropeptides in Africanized Honeybee ( Apis mellifera) Brain: Effect of Aggressiveness

Journal of Proteome Research
Marcel PratavieiraMario Sergio Palma

Abstract

Aggressiveness in honeybees seems to be regulated by multiple genes, under the influence of different factors, such as polyethism of workers, environmental factors, and response to alarm pheromones, creating a series of behavioral responses. It is suspected that neuropeptides seem to be involved with the regulation of the aggressive behavior. The role of allatostatin and tachykinin-related neuropeptides in honeybee brain during the aggressive behavior is unknown, and thus worker honeybees were stimulated to attack and to sting leather targets hung in front of the colonies. The aggressive individuals were collected and immediately frozen in liquid nitrogen; the heads were removed and sliced at sagittal plan. The brain slices were submitted to MALDI spectral imaging analysis, and the results of the present study reported the processing of the precursors proteins into mature forms of the neuropeptides AmAST A (59-76) (AYTYVSEYKRLPVYNFGL-NH2), AmAST A (69-76) (LPVYNFGL-NH2), AmTRP (88-96) (APMGFQGMR-NH2), and AmTRP (254-262) (ARMGFHGMR-NH2), which apparently acted in different neuropils of the honeybee brain during the aggressive behavior, possibly taking part in the neuromodulation of different aspects of this complex behavior. Th...Continue Reading

References

Feb 18, 1998·Current Opinion in Neurobiology·D H Edwards, E A Kravitz
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·J MillorS Camazine
Feb 17, 2000·Annals of the New York Academy of Sciences·W G BendenaS S Tobe
Jul 6, 2000·Insect Biochemistry and Molecular Biology·L I GilbertR M Roe
Mar 10, 2001·Current Opinion in Neurobiology·T R Insel, L J Young
Dec 4, 2003·Annual Review of Entomology·Michael D BreedGreg J Hunt
Mar 5, 2004·Cell and Tissue Research·Hideaki TakeuchiTakeo Kubo
May 24, 2005·Cell and Tissue Research·Salvador Hernández-MartínezFernando G Noriega
Jul 8, 2005·PLoS Biology·Sean B Carroll
Nov 18, 2005·Molecular and Cellular Neurosciences·Asa M E WintherAlberto Ferrús
Sep 14, 2006·Annual Review of Entomology·Barbara Stay, Stephen S Tobe
Oct 31, 2006·Progress in Neurobiology·Frank HauserCornelis J P Grimmelikhuijzen
Oct 31, 2006·Nature·UNKNOWN Honeybee Genome Sequencing Consortium
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Miguel CoronaGene E Robinson
May 13, 2008·Journal of the American Society for Mass Spectrometry·Erin H SeeleyRichard M Caprioli
Jan 31, 2009·Proceedings of the National Academy of Sciences of the United States of America·Axel BrockmannJonathan V Sweedler
May 22, 2009·Annals of the New York Academy of Sciences·R J Weaver, N Audsley
Jul 7, 2009·Neuropharmacology·Bart BoerjanPeter Verleyen
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cédric AlauxGene E Robinson
Sep 29, 2009·Peptides·Tom Van LoyJozef Vanden Broeck
Oct 10, 2009·Insect Biochemistry and Molecular Biology·Jan A Veenstra
Sep 10, 2010·Frontiers in Systems Neuroscience·Jürgen RybakRandolf Menzel
Nov 26, 2010·Peptides·Geoffrey M Coast, David A Schooley
Nov 26, 2010·Proceedings. Biological Sciences·Jan RillichPaul A Stevenson
May 3, 2011·PloS One·David S KhouryAndrew B Barron
Jan 17, 2014·Frontiers in Behavioral Neuroscience·Stevanus R TedjakumalaMartin Giurfa
Apr 20, 2014·Journal of Proteome Research·Marcel PratavieiraMario Sergio Palma
Dec 6, 2014·Trends in Neurosciences·L Felipe BarrosBruno Weber
Feb 3, 2015·Genes, Brain, and Behavior·S ChandrasekaranG E Robinson
Jul 1, 2015·Scientific Reports·Julie L HentzeKim F Rewitz
Jan 1, 2012·Insects·Naïla EvenAndrew B Barron
Dec 24, 2015·Nature Communications·Morgane NouvianJudith Reinhard
Nov 18, 2016·The Journal of Experimental Biology·Morgane NouvianMartin Giurfa

❮ Previous
Next ❯

Citations

Nov 2, 2019·Archives of Insect Biochemistry and Physiology·Fei-Ying YangMin-Sheng You
Nov 5, 2019·Journal of Neuroendocrinology·Kelly J RobinsonMike Ludwig
Dec 24, 2018·Expert Review of Proteomics·Samantha Louise EdwardsLiliane Schoofs
Mar 2, 2021·Frontiers in Endocrinology·Celia G BarredoFrancisco A Martin
May 6, 2021·The Journal of Comparative Neurology·Jens HabensteinWolfgang Rössler
Aug 11, 2021·Expert Review of Proteomics·Christopher S SauerLingjun Li
Nov 18, 2020·Current Protein & Peptide Science·Nhu Q VuLingjun Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.