PMID: 20631433Jul 16, 2010Paper

MALDI mass spectrometry using 2,4,6-trihydroxyacetophenone and 2,4-dihydroxyacetophenone with cyclodextrins: suppression of matrix-related ions in low-molecular-weight region

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Takashi FujitaTakashi Korenaga

Abstract

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of a model peptide, Substance P (SubP), was carried out using 2,4,6-trihydroxyacetophenone (THAP) and 2,4-dihydroxyacetophenone (DHAP) with cyclodextrins (cyclodextrin-supported matrix). It was found that the use of a cyclodextrin-supported matrix simplified the mass spectrum in the low-molecular-weight region. The interaction between THAP/DHAP and cyclodextrin (CD) was studied by UV-vis absorption spectroscopy and the incorporation of matrix molecules into the cyclodextrin cavity was confirmed by (1)H-NMR spectroscopy. DHAP showed tight incorporation with betaCD (betaCD(DHAP)) rather than THAP and it was found that the matrix-related peaks could be weakened by less than one third of the peak intensity of a protonated analyte. The betaCD(DHAP) matrix was applied to the measurements of two low-molecular-weight compounds; adenosine and adrenaline. It became clear that the cyclic structure of the CD and the host-guest interaction between betaCD and the matrix molecule were important to reduce the matrix-related peaks of THAP and DHAP.

References

Jan 15, 1999·Journal of the American Society for Mass Spectrometry·J M Asara, J Allison
Feb 28, 2004·Analytical Chemistry·Xiaofeng YangRichard B van Breemen
Aug 27, 2004·Journal of Mass Spectrometry : JMS·Ryuichi ArakawaShoji Okuno
Sep 18, 2004·Analytical Chemistry·Sven Kjellström, Ole Nørregaard Jensen
Nov 11, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sohta YamaguchiTakashi Korenaga

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Citations

Apr 25, 2012·Analytical and Bioanalytical Chemistry·Andreas SchinkovitzPascal Richomme
Jun 13, 2014·International Journal of Molecular Sciences·Lin LiZhenwen Zhao
Oct 9, 2015·International Journal of Analytical Chemistry·Mengrui Yang, Tatsuya Fujino
Sep 15, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Junya Suzuki, Tatsuya Fujino
Aug 27, 2015·European Journal of Mass Spectrometry·Kazuaki OharaKentaro Yamaguchi
Feb 14, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shu TairaHajime Katano
Oct 5, 2017·Analytical and Bioanalytical Chemistry·Ali JaberAndreas Schinkovitz

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