MALDI MS sample preparation by using paraffin wax film: systematic study and application for peptide analysis

Analytical Chemistry
Junhua WangLingjun Li

Abstract

Recently developed sample preparation techniques employing hydrophobic sample support have improved the detection sensitivity and mass spectral quality of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). These methods concentrate the samples on target by minimizing the sample area via the solvent repellent effect of the target surface. In the current study, we employed the use of paraffin wax film (Parafilm M) for improved MALDI MS analysis of low-abundance peptide mixtures, including neuronal tissue releasate and protein tryptic digests. This thin film was found to strongly repel polar solvents including water, methanol, and acetonitrile, which enabled the application of a wide range of sample preparation protocols that involved the use of various organic solvents. A "nanoliter-volume deposition" technique employing a capillary column has been used to produce tiny ( approximately 400 microm) matrix spots of 2,5-dihydroxybenzoic acid on the film. By systematically optimizing the sample volume, solvent composition, and film treatment, the Parafilm M substrate in combination with the nanoliter-volume matrix deposition method allowed dilute sample to be concentrated on the film for MALDI MS analysis. Pepti...Continue Reading

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Citations

Oct 25, 2011·Analytical Chemistry·Kevin R TuckerJonathan V Sweedler
Feb 19, 2009·Journal of Proteome Research·Mingming MaLingjun Li
Nov 30, 2014·Applied Biochemistry and Biotechnology·Anna Smolira, Joanna Wessely-Szponder
Feb 18, 2010·Trends in Analytical Chemistry : TRAC·Tingting Tu, Michael L Gross
Sep 22, 2018·Journal of Separation Science·Ivan Mikšík
Dec 5, 2009·Electrophoresis·Michael W VannattaNorman J Dovichi
Apr 11, 2009·Journal of Separation Science·Bryan R Fonslow, John R Yates
Jan 22, 2011·Mass Spectrometry Reviews·Pawel L UrbanRenato Zenobi
Jul 17, 2019·Scientific Reports·Ravi MawalePetr Němec
Feb 25, 2009·The Analyst·Wei-Han Wang, Merlin L Bruening
Aug 2, 2014·Current Protocols in Protein Science·Wendy Sandoval
Jan 7, 2017·Journal of the American Society for Mass Spectrometry·Sheng WangXinhua Guo

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