MALDI-TOF mass spectrometry - a rapid method for the identification of dermatophyte species

Medical Mycology
Pietro NenoffY Gräser

Abstract

Altogether 285 dermatophyte isolates of 21 different species - including both Trichophyton rubrum and T. interdigitale, but also eight additional Trichophyton species, Microsporum canis and seven other Microsporum species, as well as Epidermophyton floccosum and Arthroderma spp. - were analyzed using Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) and the AnagnosTec 'SARAMIS' (Spectral Archiving and Microbial Identification System) software. In addition, sequence analysis of the internal transcribed spacer (ITS) of the ribosomal DNA was performed for a high number of the tested strains. Sufficient agreement was found between the results obtained with standard identification methods and those with the MALDI-TOF MS for species identification of dermatophytes. A mass spectra database was constructed which contained the species identifications of all 285 isolates. The results were confirmed for 164 of the isolates by sequence analysis of the internal transcribed spacer (ITS) of the ribosomal DNA. Statistical analysis of all 285 dermatophyte strains showed that conventional identification matched the results of MALDI-TOF MS for 78.2% of the isolates tested. In the case of the 164 isolates ...Continue Reading

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Citations

Feb 18, 2014·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·P NenoffJ C Simon
Apr 11, 2014·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·P NenoffW Handrick
May 24, 2013·Mycopathologia·Sadegh Nouripour-SisakhtLeila Hosseinpour
Mar 26, 2013·Enfermedades infecciosas y microbiología clínica·Leonel PereiraNelson Lima
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Apr 15, 2015·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·P NenoffP Mayser
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Jan 10, 2018·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Silke UhrlaßPietro Nenoff

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