MALDI-TOF mass spectrometry: a powerful tool to study the internalization of cell-penetrating peptides

Biochimica Et Biophysica Acta
Soline AubryFabienne Burlina

Abstract

This review summarizes the contribution of MALDI-TOF mass spectrometry in the study of cell-penetrating peptide (CPP) internalization in eukaryote cells. This technique was used to measure the efficiency of cell-penetrating peptide cellular uptake and cargo delivery and to analyze carrier and cargo intracellular degradation. The impact of thiol-containing membrane proteins on the internalization of CPP-cargo disulfide conjugates was also evaluated by combining MALDI-TOF MS with simple thiol-specific reactions. This highlighted the formation of cross-linked species to cell-surface proteins that either remained trapped in the cell membrane or led to intracellular delivery. MALDI-TOF MS is thus a powerful tool to dissect CPP internalization mechanisms.

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Aug 21, 2013·PloS One·Sofie StalmansBart De Spiegeleer
May 15, 2013·FEBS Letters·Chérine Bechara, Sandrine Sagan
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Dec 18, 2020·International Journal of Toxicology·Doris ZaneJessica Hawes
Nov 28, 2017·Accounts of Chemical Research·Astrid WalrantSandrine Sagan

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