Mar 9, 2019

MALDI-TOF MS-based total serum protein fingerprinting for liver cancer diagnosis

The Analyst
Han-Gyu ParkYun-Gon Kim

Abstract

Serum is one of the most commonly used samples in many studies to identify protein biomarkers to diagnose cancer. Although conventional enzyme-linked immunosorbent assay (ELISA) or liquid chromatography-mass spectrometry (LC-MS)-based methods have been applied as clinical tools for diagnosing cancer, there have been troublesome problems, such as inferior multiplexing capabilities, high development costs and long turnaround times, which are inappropriate for high-throughput analytical platforms. Here, we developed a simple and robust cancer diagnostic method using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based total serum protein fingerprinting. First, serum samples were simply diluted with distilled water and subsequently spotted onto a MALDI plate without prior chromatographic purification or separation. The sample preparation method was enough to collect reproducible total serum protein fingerprints and would be highly advantageous for high-throughput assay. Each of the integrated main spectrum profiles (MSPs), which are representative of liver cancer patients (n = 40) or healthy controls (n = 80), was automatically generated by the MALDI Biotyper 3 software. The reliability ...Continue Reading

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Mentioned in this Paper

Biological Markers
Study
High Throughput Screening
Distilled water
CTSC
Evaluation
Protein Purification
Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry
Cancer Diagnostic Procedure
Liquid Chromatography Mass Spectrometry

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