MALDI-TOF MS identification of ticks of domestic and wild animals in Algeria and molecular detection of associated microorganisms

Comparative Immunology, Microbiology and Infectious Diseases
Mehdi BoucheikhchoukhPhilippe Parola

Abstract

Recent studies have reported the reliability of MALDI-TOF MS for arthropod identification, including fresh or alcohol-preserved ticks based on leg-derived mass spectra. The aim of this study was to evaluate the performance of MALDI-TOF MS for the identification of alcohol-preserved Algerian ticks collected from different domestic and wild hosts. Secondly, we conducted a molecular survey to detect the presence of bacterial DNA in all ticks that were previously subjected to MALDI-TOF MS. A total of 2635 ixodid and 1401 argasid ticks belonging to 9 distinct species were collected in nine different regions of northeastern Algeria. The legs of 230 specimens were subjected to MALDI-TOF MS assays. Spectral analysis revealed intra-species similarity and inter-species specificity for the MS spectra, which was consistent with the morphological identification. Blind tests against the in-lab database revealed that 93.48% of the tested specimens were correctly identified. The accuracy of the morphological and MALDI-TOF MS identifications was validated by sequencing the 12S ribosomal RNA gene (rRNA) for 33 specimens and all the ticks were correctly identified. The quantitative PCR screening showed that for 219 tested ticks, 15 were positive ...Continue Reading

Citations

May 1, 2020·Parasite : Journal De La Société Française De Parasitologie·Basma OuartiPhilippe Parola
Oct 3, 2018·Parasitology·Andrea L LawrenceMaureen Laroche
Nov 13, 2019·The Onderstepoort Journal of Veterinary Research·Rosa KebbiAbdelhanine Ayad
Sep 15, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Linda BenkacimiMaureen Laroche
Sep 29, 2021·PLoS Neglected Tropical Diseases·Ly Na HuynhPhilippe Parola
Oct 13, 2021·Parasites & Vectors·Roland BamouChristophe Antonio-Nkondjio

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