MALDI-TOF MS: optimization for future uses in entomological surveillance and identification of mosquitoes from New Caledonia.

Parasites & Vectors
Antsa RakotonirinaNicolas Pocquet

Abstract

Mosquito vectors cause a significant human public health burden through the transmission of pathogens. Due to the expansion of international travel and trade, the dispersal of these mosquito vectors and the pathogens they carry is on the rise. Entomological surveillance is therefore required which relies on accurate mosquito species identification. This study aimed to optimize the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for mosquito identification. Aedes aegypti of the Bora-Bora strain and 11 field-sampled mosquito species were used in this study. Analyses were performed to study the impact of the trapping duration on mosquito identification with MALDI-TOF MS. The best preservation methods to use for short, medium and long-term preservation before MALDI-TOF MS analysis were also assessed. In addition, the number of specimens per species required for MALDI-TOF MS database creation was determined. The first MALDI-TOF database of New Caledonian mosquitoes was assembled and the optimal threshold for mosquito species identification according to the sensitivity and specificity of this technique was determined. This study showed that the identification scores decreased as the ...Continue Reading

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Citations

Jan 23, 2021·Medical and Veterinary Entomology·L C de Sousa-PaulaF Dantas-Torres
Jan 11, 2021·Malaria Journal·Cécile NabetRenaud Piarroux

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Datasets Mentioned

BETA
MN733743-MN733814

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
GAP
PREGAP
R
MALDI Biotyper
R Core Team
MALDI flex
MALDI Biotyper compass
flexControl

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