MALDI-TOF MS Supplementary database for species identification employing the yeast diversity encountered on southern Brazil grapes

Folia Microbiologica
Bruna Carla AgustiniTania M B Bonfim

Abstract

The study of grape microflora is of interest when autochthonous yeasts, which are related to typical wine characteristics, are intended to be used in winemaking. The election of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) as the first method for yeast identification was based on its accuracy and rapidity compared to alternative laboratory protocols for identification. The aims of this study are to consolidate the MALDI-TOF MS Supplementary database for environmental yeasts already constructed, to expand it through the addition of standard spectra of not included yeast species, and to discuss the grape microflora encountered in Southern Brazil. A total of 358 strains, isolated from grape berries, were submitted to protein profiling employing Biotyper and Supplementary database. Molecular biology techniques were used as alternatives to identify 6.4% of strains not promptly designated by protein profiling. These strains corresponded to the species Candida californica, Zygoascus meyerae, Candida akabanensis, Candida azyma, and Hanseniaspora vineae. The MALDI-TOF MS spectra of the identified species were added to Supplementary database. The presented results strengthen the need for fur...Continue Reading

References

Feb 24, 1999·International Journal of Systematic Bacteriology·B Esteve-ZarzosoA Querol
Aug 2, 2003·International Journal of Food Microbiology·Graham H Fleet
May 10, 2005·International Journal of Systematic and Evolutionary Microbiology·Maudy Th SmithA W A M de Cock
May 10, 2005·International Journal of Systematic and Evolutionary Microbiology·Yuka NagatsukaTatsuji Seki
Nov 3, 2007·Journal of Applied Microbiology·A BarataV Loureiro
Nov 30, 2007·Molecular & Cellular Proteomics : MCP·Sen-Yung HsiehJen-Kun Chen
Feb 23, 2008·Rapid Communications in Mass Spectrometry : RCM·Martin StrohalmMilan Kodícek
Aug 30, 2008·International Journal of Food Microbiology·Marzia StringiniMaurizio Ciani
Sep 17, 2008·FEMS Yeast Research·Graham H Fleet
Oct 20, 2009·Food Microbiology·Pradnya ChavanMukund V Deshpande
Apr 19, 2011·International Journal of Food Microbiology·J Bautista-GallegoF N Arroyo-López
Dec 23, 2011·International Journal of Food Microbiology·A BarataV Loureiro
Oct 30, 2013·FEMS Yeast Research·Neil P JollyIsak S Pretorius
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicholas A BokulichDavid A Mills
Jan 21, 2014·World Journal of Microbiology & Biotechnology·Savitree LimtongHiroko Kawasaki
Apr 2, 2014·Applied Microbiology and Biotechnology·Bruna Carla AgustiniGildo Almeida da Silva
Apr 9, 2015·Frontiers in Plant Science·Angela Mehta, Luciano P Silva
Jun 1, 2016·Frontiers in Microbiology·Carla JaraJaime Romero

❮ Previous
Next ❯

Datasets Mentioned

BETA
KX421391

Methods Mentioned

BETA
PCR
restriction profiles

Software Mentioned

MALDI Biotyper
Biotyper
Blast
mMass
BlastN
FlexAnalysis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.