MALDI-TOF to compare polysaccharide profiles from commercial health supplements of different mushroom species

Food Chemistry
Marta López-GarcíaMaría Victoria González Rodríguez

Abstract

In this work MALDI-TOF mass spectroscopy was investigated to characterise the β-glucan profiles of several commercial health supplements, without any derivatisation or purification pre-treatment. The effect of two solvents (water and dimethyl sulfoxide) and two MALDI matrices (2,5-dihydroxybenzoic acid and 2',4',6'-trihydroxyacetophenone) was first evaluated on dextran standards. MALDI-TOF was found as a useful and quick technique to obtain structural information of diverse food supplements based on mushroom extracts. The MALDI polysaccharide profiles of 5 supplements from different mushroom species were qualitatively similar showing [Glucan+Na](+) cations with a peak-to-peak mass difference of 16 Da consistent with the repeating unit of the β-(1→3)-glucan. The profiles strongly depended on the sample solvent used, with m/z values around 5000-8000 for water and 2000 for dimethyl sulfoxide; differences between samples were revealed in the molecular weight of the aqueous preparation, with the highest values for Maitake and Cordyceps species.

References

Dec 18, 2001·Rapid Communications in Mass Spectrometry : RCM·M J DeeryC G Chappell
Feb 13, 2003·Chemical Reviews·Klaus Dreisewerd
Feb 17, 2005·Journal of Agricultural and Food Chemistry·Cheryl L DikemanGeorge C Fahey
Jun 5, 2007·Rapid Communications in Mass Spectrometry : RCM·Nien-Yeen HsuChung-Hsuan Chen
Sep 17, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei-Ting HungWen-Bin Yang
Nov 13, 2009·Nutrition Reviews·Otakar RopTunde Jurikova
May 2, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei-Ting HungWen-Bin Yang
Mar 14, 2013·Journal of Chemical Information and Modeling·Luc Patiny, Alain Borel

❮ Previous
Next ❯

Citations

Nov 5, 2016·International Journal of Biological Macromolecules·Rodell C BarrientosAnamy Ma C Paano
Oct 1, 2017·Journal of the Science of Food and Agriculture·Sen YaoYuanZhong Wang
Nov 13, 2018·International Journal of Biological Macromolecules·A Hauser-KawaguchiL G Luyt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.