Male and female roles in crosses of Aspergillus nidulans as revealed by vegetatively incompatible parents

Fungal Genetics and Biology : FG & B
Judith BruggemanR F Hoekstra

Abstract

To resolve the role of male and female nuclei and mitochondria in cleistothecium formation in the model organism Aspergillus nidulans, we analysed the genetic constituents of cleistothecia from crosses between vegetatively compatible and incompatible parents. We used markers that enabled us to determine the nuclear genotype of the cleistothecial wall and the nuclear and mitochondrial genotype of the ascospores. In compatible parents, nuclear genomes and cytoplasm usually mix in the vegetative hyphae prior to the formation of the sexual stage after which any cleistothecial composition is possible. In incompatible parents, the maternal strain contributes the nuclei for the cleistothecial wall and one nucleus as well as mitochondria for the ascospore origin. The paternal strain donates one nucleus for the ascospore origin. Only in crosses between vegetatively incompatible partners, it is possible to assign a female and male role to the parental strains. Our results confirm that the vegetative heterokaryotic stage is not a prerequisite for cleistothecium formation. Using this tool, we analysed sexual sporulation mutants for male or female sterility.

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Citations

Jan 21, 2004·Fungal Genetics and Biology : FG & B·Judith BruggemanRolf F Hoekstra
Sep 19, 2006·Genetics·Richard B ToddAlex Andrianopoulos
Nov 21, 2012·Journal of Evolutionary Biology·B P S Nieuwenhuis, D K Aanen
Nov 19, 2011·FEMS Microbiology Reviews·Paul S Dyer, Céline M O'Gorman
Mar 18, 2006·Advances in Applied Microbiology·O P WardA Singh
May 13, 2010·Fungal Genetics and Biology : FG & B·M López-VillavicencioJ Dupont
Jul 16, 2013·Journal of Evolutionary Biology·M López-VillavicencioS E Schoustra
Nov 28, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Greg KorfantyJianping Xu

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