Male exposure to bisphenol a impairs spermatogenesis and triggers histone hyperacetylation in zebrafish testes

Environmental Pollution
Silvia González-RojoMaria Paz Herráez

Abstract

Bisphenol A (BPA) is an endocrine disruptor whose ubiquitous presence in the environment has been related with impairment of male reproduction. BPA can cause both transcriptomic and epigenetic changes during spermatogenesis. To evaluate the potential effects of male exposure to BPA, adult zebrafish males were exposed during spermatogenesis to doses of 100 and 2000 μg/L, which were reported in contaminated water bodies and higher than those allowed for human consumption. Fertilization capacity and survival at hatching were analysed after mating with untreated females. Spermatogenic progress was analysed through a morphometrical study of testes and apoptosis was evaluated by TUNEL assay. Testicular gene expression was evaluated by RT-qPCR and epigenetics by using ELISA and immunocytochemistry. In vitro studies were performed to investigate the role of Gper. Chromatin fragmentation and the presence of transcripts were also evaluated in ejaculated sperm. Results on testes from males treated with the highest dose showed a significant decrease in spermatocytes, an increase in apoptosis, a downregulation of ccnb1 and sycp3, all of which point to an alteration of spermatogenesis and to meiotic arrest and an upregulation of gper1 and es...Continue Reading

Citations

Jan 18, 2020·Environmental Health Perspectives·Felicia Fei-Lei Chung, Zdenko Herceg
Aug 28, 2020·Frontiers in Endocrinology·Federica CariatiIda Strina
Nov 29, 2020·BMC Biology·Joanna RutkowskaShinichi Nakagawa
Jul 13, 2019·Environmental Research·Ya MaHuizhen Zhang
Jan 29, 2021·Frontiers in Genetics·Tengfei QinXiangFen Xiao
Feb 22, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Hyekyoung Hannah You, Gwonhwa Song
Mar 5, 2021·Biological Reviews of the Cambridge Philosophical Society·Marta Lombó, Paz Herráez
Mar 7, 2021·International Journal of Molecular Sciences·Marta Lombó, María Paz Herráez
Jan 28, 2021·Toxicological Sciences : an Official Journal of the Society of Toxicology·Abishankari RajkumarBarbara F Hales
Apr 4, 2021·Aquatic Toxicology·Keng Po LaiWilliam Ka Fai Tse
Apr 30, 2021·Ecotoxicology and Environmental Safety·Jian TengXiang Shan Ji
May 21, 2021·Basic and Clinical Andrology·Jui-Hsiang HungYen-Ni Teng
Jun 15, 2021·Environmental Pollution·Enrique Escarda-CastroMarta Lombó

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