Male gametophyte-specific WRKY34 transcription factor mediates cold sensitivity of mature pollen in Arabidopsis.

Journal of Experimental Botany
Changsong ZouDiqiu Yu

Abstract

Mature pollen is very sensitive to cold stress in chilling-sensitive plants. Plant WRKY DNA-binding transcription factors are key regulators in plant responses to abiotic and biotic stresses. Previous studies have suggested that WRKY34 (At4g26440) gene might be involved in pollen viability, although the mechanism involved is unclear. In this study, it is shown that cold treatment increased WRKY34 expression in the wild type, and promoter-GUS analysis revealed that WRKY34 expression is pollen-specific. Enhanced green fluorescent protein-tagged WRKY34 was localized in the nuclei. Pollen harbouring the wrky34 allele showed higher viability than pollen with the WRKY34 allele after cold treatment. Further functional analysis indicated that the WRKY34 transcription factor was involved in pollen development regulated by the pollen-specific MIKC* class of MADS-domain transcription factors under cold stress, and cold-insensitivity of mature wrky34 pollen might be partly attributable to the enhanced expression of transcriptional activator CBFs in the mutants. Thus, the WRKY34 transcription factor negatively mediated cold sensitivity of mature Arabidopsis pollen and might be involved in the CBF signal cascade in mature pollen.

References

Aug 1, 1990·The Plant Cell·D R SmythE M Meyerowitz
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Apr 29, 2000·Trends in Plant Science·T EulgemI E Somssich
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Feb 27, 2003·Plant Molecular Biology·Jixin DongZhixiang Chen
Apr 4, 2003·Genes & Development·Viswanathan ChinnusamyJian-Kang Zhu
Jun 14, 2003·Plant Physiology·David Honys, David Twell
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Michael F. Thomashow
May 29, 2004·The Plant Journal : for Cell and Molecular Biology·Kyonoshin MaruyamaKazuko Yamaguchi-Shinozaki
Sep 1, 2004·Current Opinion in Plant Biology·Bekir Ulker, Imre E Somssich
Sep 10, 2004·Plant Molecular Biology·Sarah J GilmourMichael F Thomashow
Aug 27, 2005·PLoS Genetics·Matthew A HannahDirk K Hincha
Oct 11, 2005·The Plant Cell·Byeong-ha LeeJian-Kang Zhu
Nov 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ming LuoAbed Chaudhury
Dec 27, 2005·Nature Cell Biology·Toshiyuki MoriTsuneyoshi Kuroiwa
Jul 24, 2007·Current Opinion in Plant Biology·Thomas Eulgem, Imre E Somssich
Sep 15, 2007·Trends in Plant Science·Viswanathan ChinnusamyJian-Kang Zhu
Nov 24, 2007·Genome Biology·Wim VerelstThomas Münster
Dec 18, 2007·The Plant Cell·Tory ChhunMiyako Ueguchi-Tanaka

❮ Previous
Next ❯

Citations

May 10, 2012·Molecular Genetics and Genomics : MGG·Shengxiong HuangYongsheng Liu
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ligang ChenDiqiu Yu
Jul 20, 2012·BMC Systems Biology·Jigang WangTieliu Shi
Feb 24, 2016·Frontiers in Plant Science·Yunxie WeiKaimian Li
Feb 13, 2016·Frontiers in Plant Science·Hui SongXingjun Wang
Aug 30, 2012·Molecular Plant·Yanjuan JiangDiqiu Yu
Mar 13, 2015·Plant Reproduction·Nicholas Rutley, David Twell
Sep 16, 2014·Plant Science : an International Journal of Experimental Plant Biology·Bo LiuFeng-Ming Song
Apr 17, 2015·TheScientificWorldJournal·Aditya Banerjee, Aryadeep Roychoudhury
May 21, 2016·Frontiers in Plant Science·Hui SongXingjun Wang
Oct 30, 2016·Frontiers in Plant Science·Ashley R Smith, Dazhong Zhao
Dec 21, 2016·Journal of Integrative Plant Biology·Jingjing JiangJianhua Zhang
Jun 22, 2017·The Plant Journal : for Cell and Molecular Biology·Rihua LeiDiqiu Yu
Mar 15, 2013·International Journal of Molecular Sciences·Søren LindemoseKaren Skriver
Feb 22, 2017·Frontiers in Plant Science·Pengfei WangXingjun Wang
Feb 5, 2014·Plant Signaling & Behavior·Madhunita Bakshi, Ralf Oelmüller

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
genotyping
transgenic
Fluorescence

Software Mentioned

Mircoarray

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.