Male mouse meiotic chromosome cores deficient in structural proteins SYCP3 and SYCP2 align by homology but fail to synapse and have possible impaired specificity of chromatin loop attachment

Cytogenetic and Genome Research
N K KolasP B Moens

Abstract

The targeted deletion of the meiotic chromosome core component MmSYCP3 results in chromosome synaptic failure at male meiotic prophase, extended meiotic chromosomes, male sterility, oocyte aneuploidy and absence of the MmSYCP2 chromosome core component. To test the functions of SYCP2 and SYCP3 proteins in the cores, we determined the effect of their deletion on homology recognition by whole chromosome painting and the effect on chromatin loop attachment to the cores with endogenous and exogenous sequences. Because we observed that the alignment of cores is between homologs, it suggested that alignment is not a function of the chromosome core components but might be mediated by chromatin-chromatin interactions. The alignment function therefore appears to be separate from intimate synapsis function of homologous cores that is observed to be defective in the SYCP3-/- males. To examine the functions of the SYCP2 and 3 core proteins in chromatin loop attachment, we measured the loop sizes of the centromeric major satellite chromatin and the organization of an exogenous transgene in SYCP3+/+ and SYCP3-/- males. We observed that these satellite chromatin loops have a normal appearance in SYCP3-/- males, but the loop regulation of a 2-...Continue Reading

Citations

Mar 7, 2006·Chromosoma·Ekaterina Revenkova, Rolf Jessberger
Feb 8, 2011·Asian Journal of Andrology·Michael C HannHelen G Tempest
Jan 2, 2013·Molecular Cytogenetics·Matthew E JohnsonTerry Hassold
Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Meisha A Morelli, Paula E Cohen
May 17, 2013·Archives of Gynecology and Obstetrics·Andreas RoosSimone Heidemann
Aug 9, 2006·The Journal of Cell Biology·Da-Qiao DingYasushi Hiraoka
Jun 20, 2012·The Journal of Cell Biology·Elena LlanoAlberto M Pendás
Mar 8, 2012·Experimental Cell Research·Johanna FrauneRicardo Benavente
Dec 30, 2015·Chromosoma·J M CloutierJames Turner
Apr 17, 2007·Fertility and Sterility·Juanjo MartínezSara Larriba
May 20, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kenji SaitoNoriyoshi Sakai
Jan 15, 2013·Current Opinion in Genetics & Development·Valérie Borde, Bernard de Massy
Apr 20, 2016·Experimental Cell Research·Rosario OrtizAbrahan Hernández-Hernández
Aug 13, 2005·Progress in Nucleic Acid Research and Molecular Biology·S Anuradha, K Muniyappa
Sep 7, 2006·Cytogenetic and Genome Research·C J YeH H Q Heng
May 1, 2008·Systems Biology in Reproductive Medicine·Matthew L SandersonDouglas T Carrell
Oct 17, 2019·Open Biology·Daniel BollschweilerLuca Pellegrini
Jan 5, 2017·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Philip W JordanSusannah Rankin
Aug 7, 2019·The Biochemical Journal·Isobel A MacGregorNick Gilbert
Dec 24, 2019·American Journal of Human Genetics·Samantha L P SchilitCynthia C Morton
Oct 26, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Maryam SaberFereshteh Esfandiari

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.