Male obesity is associated with changed spermatozoa Cox4i1 mRNA level and altered seminal vesicle fluid composition in a mouse model

Molecular Human Reproduction
Natalie K BinderDavid K Gardner

Abstract

The rate of obesity among men of reproductive age has tripled in the last three decades. Previously, we demonstrated that paternal obesity resulted in impaired preimplantation developmental kinetics, compromised post-compaction metabolism and decreased blastocyst cell number when embryos were generated in vivo. Subsequently, using in vitro fertilization we found embryos of obese males to have altered metabolism before compaction, reduced inner cell mass cell number and retarded fetal development--the difference between these two studies being the method of embryo generation and the presence or absence of seminal plasma, respectively. Here, we hypothesize that both sperm and seminal plasma are affected by obesity, compromising embryogenesis and pregnancy health in a cumulative manner. Epididymal sperm and seminal vesicle fluid were collected from normal and obese C57BL/6 mice. RNA and DNA were extracted from spermatozoa for qPCR and global methylation analysis, respectively. Proteomic (Luminex) and metabolomic (GC-MS) techniques were employed to analyse the composition of seminal vesicle fluid. Nuclear encoded cytochrome c oxidase subunit IV isoform 1 (Cox4i1) of the terminal enzyme in the mitochondrial respiratory chain demonst...Continue Reading

References

Nov 1, 1980·The Journal of Clinical Endocrinology and Metabolism·H K KleyH L Krüskemper
Jul 17, 1999·International Urology and Nephrology·F T Andrade-Rocha
Jun 25, 2002·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·A GambineriR Pasquali
Nov 26, 2003·International Journal of Andrology·T JopeH-J Glander
Jan 31, 2004·American Journal of Reproductive Immunology : AJRI·Pavla ManáskováVĕra Jonáková
Sep 16, 2004·Reproduction in Domestic Animals = Zuchthygiene·I CaballeroE A Martinez
Oct 16, 2004·Fertility and Sterility·Tina Kold JensenNiels E Skakkebaek
Nov 20, 2004·Endocrinology·Saveria AquilaSebastiano Andò
Nov 30, 2004·Human Reproduction·Elin V MagnusdottirKristin Olafsdottir
Mar 5, 2005·Reproduction, Fertility, and Development·David K Gardner, Michelle Lane
Mar 5, 2005·Reproduction, Fertility, and Development·Michelle Lane, David K Gardner
Jun 10, 2005·The Journal of Clinical Endocrinology and Metabolism·Saveria AquilaSebastiano Andò
Jun 28, 2005·Insect Biochemistry and Molecular Biology·K Ravi RamM F Wolfner
Nov 4, 2005·Andrologia·I FejesA Pál
Dec 13, 2005·Journal of Andrology·Hilton I KortWilliam E Roudebush
Aug 8, 2006·Clinical Pharmacology and Therapeutics·Themba T NdoviCraig W Hendrix
Mar 16, 2007·International Journal of Andrology·Marco De AmbrogiCarlo Tamanini
Oct 31, 2007·Asian Journal of Andrology·Dan-Dan QinEr-Sheng Gao
Dec 11, 2007·Fertility and Sterility·Anette S AggerholmJens Peter Bonde
Jan 8, 2008·Fertility and Sterility·Ahmad O HammoudA Wayne Meikle
Feb 23, 2008·Fertility and Sterility·Eric M PauliPeter A Lee
Mar 28, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Saveria AquilaAmalia Carpino
Jan 29, 2009·The Journal of Clinical Endocrinology and Metabolism·Ahmad HammoudA Wayne Meikle
Jan 9, 2010·Fertility and Sterility·Ana C MartiniRubén D Ruiz
Jul 24, 2010·International Journal of Andrology·H W BakosM Lane
Nov 5, 2010·Fertility and Sterility·Megan MitchellMichelle Lane
Jan 7, 2011·Biology of Reproduction·Payal MitalJannette M Dufour
Mar 9, 2011·Andrologia·O TuncK Tremellen
Mar 15, 2011·Reproductive Biology and Endocrinology : RB&E·Carla D B FernandezWilma D G Kempinas
Jul 8, 2011·American Journal of Reproductive Immunology : AJRI·Heriberto Rodríguez-MartínezJuan J Calvete
Aug 30, 2011·Reproduction, Fertility, and Development·Nicole O PalmerMichelle Lane
Nov 1, 2011·Pharmacological Reviews·C Yan Cheng, Dolores D Mruk
Jan 19, 2012·American Journal of Physiology. Endocrinology and Metabolism·Nicole O PalmerMichelle Lane
Jan 28, 2012·Science·Michel Bornens
Feb 4, 2012·BJU International·Roberta M FarielloRenato Fraietta
Feb 15, 2012·Molecular & Cellular Proteomics : MCP·Amy J ClaydonRobert Beynon

❮ Previous
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Citations

Dec 15, 2015·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Clemens BönischJohannes Beckers
Sep 13, 2015·Current Environmental Health Reports·Haotian WuJ Richard Pilsner
Sep 1, 2016·Journal of Developmental Origins of Health and Disease·C S Rosenfeld
Oct 27, 2016·International Journal of Obesity : Journal of the International Association for the Study of Obesity·M M BroadneyE H Yeung
Mar 15, 2016·Scientific Reports·Angela B JavurekCheryl S Rosenfeld
Apr 6, 2016·Molecular & Cellular Proteomics : MCP·Laura SolerElisabeth Blesbois
Aug 30, 2016·Reproduction, Fertility, and Development·Angela B JavurekCheryl S Rosenfeld
Nov 11, 2017·Basic and Clinical Andrology·Georges RaadValérie Grandjean
Apr 26, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Cheryl S RosenfeldRex A Hess
Dec 23, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Richard M Sharpe
Sep 16, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tom P Fleming
Jan 5, 2020·Andrology·Chelsea MarchoJ Richard Pilsner
Jan 31, 2020·Physiological Reviews·John E Schjenken, Sarah A Robertson
Nov 20, 2016·Annals of the New York Academy of Sciences·Elissa Z CameronLaura M Parsley
Aug 9, 2019·International Journal of Obesity Supplements·Giuseppe BellastellaUNKNOWN Obesity Programs of nutrition, Education, Research and Assessment (OPERA) Group
Aug 8, 2019·Evolution Letters·Leigh W Simmons, Maxine Lovegrove
Feb 14, 2019·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Angela J Crean, Alistair M Senior
Jan 23, 2018·Frontiers in Medicine·Louise C Kenny, Douglas B Kell
Mar 30, 2021·Frontiers in Endocrinology·Taylor PiniAngela J Crean
Jun 3, 2021·International Journal of Molecular Sciences·Isidora M StarovlahSilvana A Andric
Jun 6, 2021·Molecular & Cellular Proteomics : MCP·David A Skerrett-ByrneJohn E Schjenken
Oct 10, 2021·BMC Genomics·David A Skerrett-ByrneJohn E Schjenken

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