Male-predominant carboxylesterase expression in the reproductive system of molluscs and insects: immunochemical and biochemical similarity between Mytilus male associated polypeptide (MAP) and Drosophila sex-specific esterase S

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
A T MikhailovJ Méndez

Abstract

We suggested that sexual differentiation of the reproductive system in gonochoric species of invertebrates can be characterized by common molecular mechanisms in spite of high morphological divergences of reproductive tract organs in different animal groups. The present study focused on this problem and report our observations on biochemical characteristics of male-associated polypeptide (MAP) identified in the gonad tissue of bivalve molluscs, Mytilus galloprovincialis, in comparison to those of male-specific carboxylesterase (esterase S) of Drosophila virilis ejaculatory bulbs. We provide evidences for the immunochemical similarity of Mytilus MAP and Drosophila esterase S. We also show that MAP is characterized by esterase activity toward both, alpha- and beta-naphthyl acetates. Using immunofluorescence, we found MAP in the gonad (mantle) connective tissue, membranes of follicles and around gonad ducts but not in sperm cells. Nevertheless, the levels of MAP expression depend on presence or absence of ripe spermatozoa in the gonad follicles. In mature gonads before spawning, MAP is expressed at high level, while in the spent gonads only traces of this polypeptide could be detected. Using Western immunoblot, MAP was not observe...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1975·Methods in Enzymology·K Scott, B Zerner
Sep 1, 1992·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·B Ozretić, M Krajnović-Ozretić
Nov 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E Kubli
Aug 11, 1995·The Journal of Biological Chemistry·B YanA Parkinson
Jan 1, 1995·Archives of Insect Biochemistry and Physiology·S WhyardV K Walker
May 1, 1995·Insect Biochemistry and Molecular Biology·J A Argentine, A A James
Jan 1, 1994·Vox Sanguinis·S M RingD M Swallow
Feb 1, 1995·Archives of Biochemistry and Biophysics·B YanA Parkinson
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Z LudwigR C Richmond
Jul 25, 1993·Nucleic Acids Research·P V SergeevG P Georgiev

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Citations

Dec 15, 2015·Molecular Reproduction and Development·Raffaele BoniElisabetta Tosti
Aug 2, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Martha KaloyianniStefanos Dailianis
Sep 4, 2009·Acta Biochimica Et Biophysica Sinica·Li ZhangYonglian Zhang

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