Male reprotoxicity associated with Sophora japonica treatment: evaluation of cellular and molecular events in vitro

Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society
Przemyslaw SolekMarek Koziorowski

Abstract

Toxicological studies are urgently needed to confirm the safety of Sophora japonica extracts for clinical use. In particular, in addition to pharmacy and pharmacology, phytotherapy, herbal medication, Sophora japonica extracts are widely used as biologically active supplements. Scientfic data suggests that some of Sophora japonica extract components have very comprehensive biological effects. In the present study, our hypothesis assumed the potential reprotoxicity of Sophora japonica extract in respect to mouse germ cells (GC-1 spg, GC-2 spd) in vitro. Specifically, we were interested in the high-performance liquid chromatography (HPLC) identification of extract components and its stress-related effects on cellular and biochemical features, such as mitochondrial metabolism, cell cycle progression, oxidative stress balance and micronuclei formation. The results indicate that Sophora japonica extract induces oxidative/nitrosative stress-mediated impairment of the mechanism for free radicals scavenging, which may provoke genotoxic events in germ cells, by cell cycle arrest and micronuclei formation. Therefore, the interplay between reactive oxygen species (ROS)/reactive nitrogen species (RNS) and antioxidant system is critical for...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.