Male-specific RNA coliphages detected by plaque assay and RT-PCR in tropical river waters and animal fecal matter

International Journal of Environmental Health Research
Stacey Yong Foong YeeYong Su Ming

Abstract

Male-specific RNA coliphages (FRNA) have been recommended as indicators of fecal contamination and of the virological quality of water. In this study, 16 river water and 183 animal fecal samples were examined for the presence of FRNA coliphages by a plaque assay using Salmonella typhimurium WG49 and WG25 to differentiate between male-specific and somatic phages, a RNase spot test to differentiate between DNA and RNA phages and a reverse transcriptase-polymerase chain reaction (RT-PCR) for the specific identification of FRNA phages. The overall recovery rate for F-specific coliphages was 8.0%. (4.4% from animal fecal matter and 50% from river water samples). Plaque counts were generally low (< 6 x 10(2) pfu per g feces or ml water), with FRNA (6.5%) and Male-specific DNA coliphages (FDNA) (7.0%) phages occurring at almost equal frequencies. The RT-PCR was positive in all FRNA plaques and was able to identify FRNA phages in mixed populations of FRNA, FDNA and somatic phages.

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Citations

Aug 4, 2009·Applied and Environmental Microbiology·Tineke H Jones, Michael W Johns
Sep 22, 2009·Applied and Environmental Microbiology·Jung Eun LeeGwangpyo Ko
May 21, 2016·Annals of the New York Academy of Sciences·Shafiqul A Sarker, Harald Brüssow
Mar 18, 2017·Letters in Applied Microbiology·B R McMinnA Korajkic
Apr 1, 2014·Clean : Soil, Air, Water·Atheesha GaneshMoganavelli Singh

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