Male sterility associated with overexpression of the noncoding hsromega gene in cyst cells of testis of Drosophila melanogaster

Journal of Genetics
T K RajendraS C Lakhotia

Abstract

Of the several noncoding transcripts produced by the hsromega gene of Drosophila melanogaster, the nucleus-limited >10-kb hsromega-n transcript colocalizes with heterogeneous nuclear RNA binding proteins (hnRNPs) to form fine nucleoplasmic omega speckles. Our earlier studies suggested that the noncoding hsromega-n transcripts dynamically regulate the distribution of hnRNPs in active (chromatin bound) and inactive (in omega speckles) compartments. Here we show that a P transposon insertion in this gene's promoter (at -130 bp) in the hsromega05421; enhancer-trap line had no effect on viability or phenotype of males or females, but the insertion-homozygous males were sterile. Testes of hsromega05421; homozygous flies contained nonmotile sperms while their seminal vesicles were empty. RNA:RNA in situ hybridization showed that the somatic cyst cells in testes of the mutant male flies contained significantly higher amounts of hsromega-n transcripts, and unlike the characteristic fine omega speckles in other cell types they displayed large clusters of omega speckles as typically seen after heat shock. Two of the hnRNPs, viz. HRB87F and Hrb57A, which are expressed in cyst cells, also formed large clusters in these cells in parallel wit...Continue Reading

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Citations

Jul 30, 2011·Journal of Biosciences·Subhash C Lakhotia
May 28, 2013·Briefings in Functional Genomics·Paulo P AmaralJohn S Mattick
Nov 3, 2010·Molecular Biology and Evolution·Zi-Feng JiangCarlos A Machado
Nov 22, 2008·Science·T A R PriceN Wedell
Apr 25, 2009·PLoS Genetics·John S Mattick
Apr 10, 2007·FEBS Letters·Alexander M BoutanaevDmitry I Nurminsky
Sep 8, 2011·Genesis : the Journal of Genetics and Development·Akanksha VermaSubhash C Lakhotia
Nov 20, 2009·Evolution; International Journal of Organic Evolution·Tom A R PriceNina Wedell
Sep 23, 2020·Non-coding RNA Research·He Chen, Ge Shan
Mar 2, 2010·Current Biology : CB·Tom A R PriceNina Wedell
Jan 25, 2011·Seminars in Cell & Developmental Biology·Michael B Clark, John S Mattick

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