Maleylated-BSA enhances production of nitric oxide from macrophages

Biochemical and Biophysical Research Communications
P B AlfordR E Shackelford

Abstract

Maleylated-bovine serum albumin (maleyl-BSA) elicits transcription and secretion of a number of proinflammatory genes via ligation of the low-affinity scavenger receptor (SR) on macrophages. We now demonstrate that while neither maleyl-BSA, nor interferon-gamma (INF-gamma) alone induce nitric oxide (NO) production, when combined they promote release of NO from murine peritoneal macrophages. This effect was blocked by treatment with oxidized-low density lipoprotein. Maleyl-BSA activated NF-kappaB dimers capable of binding the NF-kappaBd sequence unique to the iNOS promoter, but this failed to induce significant new transcription or accumulation of iNOS mRNA. The combination of maleyl-BSA and IFN-gamma failed to demonstrate synergy at the transcriptional or mRNA levels, as these levels were comparable to those elicited by IFN-gamma alone. These studies suggest that the synergy in NO production between maleyl-BSA and IFN-gamma occurs after the accumulation of iNOS-specific mRNA, possibly at the translational or post-translational level.

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Citations

Jun 27, 2006·Journal of Occupational Medicine and Toxicology·Dingena L ValstarPaul A J Henricks
Mar 7, 2015·Biological & Pharmaceutical Bulletin·Yuichi MikiYasuyuki Fujiwara
Apr 12, 2000·Microbes and Infection·P J Gough, S Gordon
Jan 23, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Hikari SatoYoshiki Katayama

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