Malignant cell-derived extracellular vesicles express different chromogranin epitopes compared to prostasomes

The Prostate
Louise DuboisK Göran Ronquist

Abstract

Prostasomes are nanosized extracellular vesicles exocytosed by prostate epithelial cells. They have been assigned many roles propitious to sperm in favor of fertilization. Prostatic cancer cells can also produce and secrete extracellular vesicles. We assessed using ELISA, the surface expression of chromogranin proproteins on prostasomes and malignant extracellular vesicles of four different prostate cancer cell-lines, two hormone sensitive and two hormone refractory. We used a panel of chromogranin A and chromogranin B antibodies against peptides in-between hypothetical cleavage sites along the proproteins. A diverging pattern of chromogranin peptides was apparent when comparing prostasomes and malignant extracellular vesicles indicating a phenotypical change. We also compared western blot patterns (prostasomes and malignant extracellular vesicles) for selected antibodies that displayed high absorbances in the ELISA. Western blot analyses revealed various cleavage patterns of those proproteins that were analyzed in prostasomes and extracellular vesicles. Chromogranins are constituents of not only prostasomes but also of malignant prostate cell-derived extracellular vesicles with different amino acid sequences exposed at the mem...Continue Reading

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Citations

Nov 29, 2015·The Urologic Clinics of North America·Jeffrey J TosoianChristian P Pavlovich
Apr 5, 2017·Prostate Cancer and Prostatic Diseases·V R MinciacchiD Di Vizio
Jan 20, 2017·Platelets·Frank A W CoumansRienk Nieuwland
Mar 10, 2016·Journal of Extracellular Vesicles·Karl Göran RonquistTheocharis Panaretakis
Nov 29, 2017·The AAPS Journal·Anjana Jeyaram, Steven M Jay

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