Malignant melanoma and bone resorption.

British Journal of Cancer
Y S LauN A Athanasou

Abstract

The cellular and humoral mechanisms accounting for osteolysis in skeletal metastases of malignant melanoma are uncertain. Osteoclasts, the specialised multinucleated cells that carry out bone resorption, are derived from monocyte/macrophage precursors. We isolated tumour-associated macrophages (TAMs) from metastatic (lymph node/skin) melanomas and cultured them in the presence and absence of osteoclastogenic cytokines and growth factors. The effect of tumour-derived fibroblasts and melanoma cells on osteoclast formation and resorption was also analysed. Melanoma TAMs (CD14+/CD51-) differentiated into osteoclasts (CD14-/CD51+) in the presence of receptor activator for nuclear factor kappaB ligand (RANKL) and macrophage-colony stimulating factor. Tumour-associated macrophage-osteoclast differentiation also occurred via a RANKL-independent pathway when TAMs were cultured with tumour necrosis factor-alpha and interleukin (IL)-1alpha. RT-PCR showed that fibroblasts isolated from metastatic melanomas expressed RANKL messenger RNA and the conditioned medium of cultured melanoma fibroblasts was found to be capable of inducing osteoclast formation in the absence of RANKL; this effect was inhibited by the addition of osteoprotegerin (OPG...Continue Reading

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Citations

Jun 27, 2015·International Journal of Surgical Pathology·Jason K WassermanYasmine Ayroud
May 30, 2007·British Journal of Cancer·Y S LauN A Athanasou
Jan 22, 2008·Journal of the American Academy of Dermatology·Roger H Weenig
Jul 12, 2017·The American Journal of Dermatopathology·Xiaoxiao LiDonald A Glass
Dec 8, 2006·Breast Cancer Research and Treatment·Y S LauN A Athanasou
Dec 12, 2007·Cancer Metastasis Reviews·Karen M BussardAndrea M Mastro
Oct 27, 2010·Surgical Neurology International·Nunung Nur RahmahKazuhiro Hongo
Feb 10, 2007·Expert Review of Anticancer Therapy·M Baud'huinM Padrines
Nov 19, 2015·Clinical Reviews in Allergy & Immunology·A SabokbarN A Athanasou
Feb 22, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Juan WangCheng Qian
Oct 5, 2017·Scientific Reports·Vardit KramMarian F Young
Aug 14, 2020·Dermatologic Therapy·Antonio CaldariaClaudio Conforti

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
density gradient centrifugation
light microscopy
PCR

Software Mentioned

Adobe Photoshop
ImageJ

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