Malleability of human skeletal muscle Na(+)-K(+)-ATPase pump with short-term training

Journal of Applied Physiology
H J GreenJ Ouyang

Abstract

To investigate the hypothesis that short-term submaximal training would result in changes in Na(+)-K(+)-ATPase content, activity, and isoform distribution in skeletal muscle, seven healthy, untrained men [peak aerobic power (peak oxygen consumption; Vo(2 peak)) = 45.6 ml x kg(-1) x min(-1) (SE 5.4)] cycled for 2 h/day at 60-65% Vo(2 peak) for 6 days. Muscle tissue, sampled from the vastus lateralis before training (0 days) and after 3 and 6 days of training and analyzed for Na(+)-K(+)-ATPase content, as assessed by the vanadate facilitated [(3)H]ouabain-binding technique, was increased (P < 0.05) at 3 days (294 +/- 8.6 pmol/g wet wt) and 6 days (308 +/- 15 pmol/g wet wt) of training compared with 0 days (272 +/- 9.7 pmol/g wet wt). Maximal Na(+)-K(+)-ATPase activity as evaluated by the 3-O-methylfluorescein phosphatase assay was increased (P < 0.05) by 6 days (53.4 +/- 5.9 nmol x h(-1) x mg protein(-1)) but not by 3 days (35.9 +/- 4.5 nmol x h(-1) x mg protein(-1)) compared with 0 days (37.8 +/- 3.7 nmol x h(-1) x mg protein(-1)) of training. Relative isoform distribution, measured by Western blot techniques, indicated increases (P < 0.05) in alpha(2)-content by 3 days and beta(1)-content by 6 days of training. These results in...Continue Reading

References

Sep 11, 1986·Biochimica Et Biophysica Acta·K KjeldsenT Clausen
Feb 1, 1973·Analytical Biochemistry·G R Schacterle, R L Pollack
Jan 1, 1967·Scandinavian Journal of Clinical and Laboratory Investigation·J Bergström, E Hultman
Oct 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·A NørgaardT Clausen
Jan 1, 1983·Annual Review of Physiology·N J Christensen, H Galbo
Jun 1, 1993·The American Journal of Physiology·H J GreenD Ranney
Mar 1, 1996·Acta Physiologica Scandinavica·A A McDonough, C B Thompson
May 1, 1997·The American Journal of Physiology·F EvertsenK Nicolaysen
May 2, 1998·Analytical Biochemistry·S F Fraser, M J McKenna
Nov 14, 1998·The American Journal of Physiology·G Blanco, R W Mercer
May 8, 1999·Journal of Applied Physiology·H GreenN L Melissa
Jun 11, 1999·The American Journal of Physiology·C B ThompsonA A McDonough
Jul 17, 1999·The American Journal of Physiology·H GreenD Ranney
Jan 15, 2000·The Journal of Biological Chemistry·G CrambertK Geering
Apr 6, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C JuelJ Bangsbo
Oct 4, 2000·Physiological Reviews·O M Sejersted, G Sjøgaard
Feb 13, 2001·American Journal of Physiology. Heart and Circulatory Physiology·D M HallC V Gisolfi
Aug 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S HeJ B Lingrel
Oct 17, 2002·Journal of Applied Physiology·Steve F FraserMichael J McKenna
Oct 17, 2002·Journal of Applied Physiology·J R FowlesA R Tupling
Jun 14, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yuk-Chow NgRussell L Moore
Jul 29, 2003·Journal of Applied Physiology·J R FowlesJ Ouyang

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Citations

Oct 22, 2010·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Howard J GreenSusan Tupling
Nov 18, 2011·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Howard J GreenSusan Tupling
Apr 5, 2012·Canadian Journal of Physiology and Pharmacology·Howard J GreenSusan Tupling
Apr 5, 2012·Canadian Journal of Physiology and Pharmacology·Howard J GreenSusan Tupling
Sep 25, 2010·Scandinavian Journal of Medicine & Science in Sports·F M Iaia, J Bangsbo
Jul 6, 2018·Medicine and Science in Sports and Exercise·Eduardo Martinez-ValdesDeborah Falla
Jun 18, 2011·American Journal of Physical Medicine & Rehabilitation·Howard J GreenJing Ouyang
Feb 12, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nikolai NordsborgJens Bangsbo
Dec 30, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Magni MohrJens Bangsbo
Dec 21, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·F Marcello IaiaJens Bangsbo
Jan 31, 2008·American Journal of Physiology. Endocrinology and Metabolism·H J GreenJ Ouyang
Jan 26, 2012·American Journal of Physiology. Endocrinology and Metabolism·Hanneke BoonAlexander V Chibalin
May 24, 2012·Medicine and Science in Sports and Exercise·Thomas Petursson GunnarssonJens Bangsbo
Feb 18, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martin Krøyer RasmussenNikolai B Nordsborg
Sep 10, 2011·Medicine and Science in Sports and Exercise·Carolina Vila-ChãDario Farina
Jan 27, 2018·Journal of Strength and Conditioning Research·David T KellyNiall M Moyna
Jun 30, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D J MahoneyM A Tarnopolsky
Feb 12, 2005·Journal of Applied Physiology·Kirsten A BurgomasterMartin J Gibala
Sep 24, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K T MurphyM J McKenna
Oct 27, 2007·Journal of Applied Physiology·Michael J McKennaJean-Marc Renaud
Oct 1, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martin ThomassenNikolai B Nordsborg
Jun 6, 2020·European Journal of Applied Physiology·Muath M AltarawnehMichael J McKenna
May 10, 2007·American Journal of Physiology. Endocrinology and Metabolism·H J GreenA R Tupling
Mar 26, 2011·Journal of Applied Physiology·F Marcello IaiaJens Bangsbo
Jun 4, 2021·Medicine and Science in Sports and Exercise·Nicki Winfield AlmquistBent R Rønnestad

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