Mallory body formation by ethanol feeding in drug-primed mice

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Z Q Zhang-GouillonSamuel W French

Abstract

Drug-primed mice, created by a 5-month feeding of diethyl-1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate (DDC), followed by a 1-month withdrawal, were refed ethanol or isocaloric dextrose (control) diets intragastrically for 7 days. The formation of Mallory bodies (MBs) was monitored by immunofluorescence and immunoperoxidase microscopy using antibodies to cytokeratin and ubiquitin, and also by electron microscopy. The changes in cytokeratin 55 (CK55), ubiquitin conjugate, nuclear factor kappaB (NFkappaB) p65, NFkappaB p50, inhibitor kappaB alpha, c-myc, tumor necrosis factor alpha, and cytochrome P450 2E1 (CYP2E1) contents were determined by Western blotting using appropriate antibodies. The messenger RNA (mRNA) for CYP2E1, cytokeratin, ubiquitin, hepatocyte growth factor activator, and tissue transglutaminase was quantitated. MBs were present at 5 to 7 days' postfeeding with ethanol, but not with dextrose. They developed in clusters of "empty hepatocytes," where the cytokeratin antibody failed to recognize the typical filament structures seen in normal hepatocytes. MBs were larger and more numerous in the subcapsular region. Northern blots showed that CK55 mRNA was decreased by the ethanol treatment, but protein levels...Continue Reading

References

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Citations

Apr 25, 2003·Experimental and Molecular Pathology·N E RileyS W French
Apr 27, 2000·Journal of Hepatology·H DenkK Zatloukal
Oct 7, 2006·Current Opinion in Gastroenterology·J H Lefkowitch
Jan 12, 2000·Alcoholism, Clinical and Experimental Research·S BokkalaS K Joseph
May 11, 1999·Alcoholism, Clinical and Experimental Research·M TakagiK Ohkawa
Jun 8, 2001·Alcoholism, Clinical and Experimental Research·P de la M HallJ C Bode
Mar 2, 2002·Journal of Clinical Gastroenterology·Arthur J McCullough
Mar 29, 2012·Expert Opinion on Drug Metabolism & Toxicology·Christoph H Österreicher, Michael Trauner
May 29, 2007·Experimental Cell Research·Kurt ZatloukalM Bishr Omary
May 29, 2007·Experimental and Molecular Pathology·Fawzia Bardag-GorceSamuel W French
Jul 18, 2009·Proteomics·Marie-Pierre Bousquet-DubouchFawzia Bardag-Gorce
Dec 22, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jun LiSamuel William French
Dec 25, 2010·Annals of the New York Academy of Sciences·Ina BergheimKatja Breitkopf-Heinlein
Nov 26, 1998·Experimental and Molecular Pathology·Q X YuanS W French
Oct 13, 2006·Experimental and Molecular Pathology·Fawzia Bardag-GorceSamuel W French
Feb 15, 2001·Experimental and Molecular Pathology·F Bardag-GorceS W French
Dec 22, 1999·The American Journal of Physiology·H KonoR G Thurman
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M YinR G Thurman
Dec 22, 1999·The American Journal of Physiology·N O KuM B Omary

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