Malnutrition-induced macrophage apoptosis

Surgery
D E RivadeneiraJ M Daly

Abstract

Human and murine studies suggest protein-calorie malnutrition (PCM) results in significant host immunosuppression resulting in increased morbidity and mortality. Apoptosis has been implicated as an important mediator in the immunosuppression observed in several disease states. This study was designed to characterize macrophage apoptosis in a murine model of PCM and investigate components that regulate the apoptotic process, such as protein kinase C (PKC) and Bcl-2 activity. Swiss-Webster mice (n = 50) were randomly assigned to receive either a control (24% protein) or a PCM diet (0% protein) for 7 days. Peritoneal macrophages were harvested and detection of apoptosis was performed by terminal deoxy-transferase-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) and propidium iodide DNA staining under baseline and pro-apoptotic conditions. Pro-apoptotic conditions included cells treated with tumor necrosis factor-alpha (TNF-alpha) (10 ng/mL), interferon-gamma (IFN-gamma) (10 ng/mL), and a combination of both agents. In addition, levels of PKC activity and expression of Bcl-2 and p53 protein were measured. Peritoneal macrophages from PCM mice had a significantly greater amount of apoptosis at baseline and under st...Continue Reading

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Citations

Oct 4, 2005·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jianming WangShengquan Zou
Aug 8, 2007·Archives of Pharmacal Research·Eun-Wha SonSuhkneung Pyo
Feb 27, 2008·World Journal of Gastroenterology : WJG·Varut LohsiriwatNarong Lert-Akayamanee
Oct 21, 2010·The British Journal of Surgery·M KandaA Nakao
Jul 7, 2015·The Lancet Infectious Diseases·Yuichiro ShindoUNKNOWN Central Japan Lung Study Group
Jul 1, 2015·The Journal of Surgical Research·Tomoyuki MoriyaJunji Yamamoto
Dec 8, 2010·Vaccine·Hyuk-Joon Kwon, Sun-Hee Cho
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Oct 13, 2017·Nutrition Reviews·Ed W SantosPrimavera Borelli
Mar 4, 2003·The Journal of Nutrition·Bill Woodward
Jan 15, 2019·World Journal of Clinical Cases·Narongsak RungsakulkijSuraida Aeesoa

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