Malorientation and abnormal segregation of chromosomes during recovery from colcemid and nocodazole

Cell Motility and the Cytoskeleton
K S Ladrach, James R LaFountain

Abstract

Reversal of meiotic arrest in crane-fly spermatocytes by U. V. irradiation of Colcemid-arrested cells or by rinsing Nocodazole-arrested cells in fresh buffer results in the induction of chromosome malorientation. Malorientations observed among Colcemid-recovering and Nocodazole-recovering spermatocytes at frequencies higher than normally observed in untreated cells included associations of sister kinetochores of half-bivalents with both spindle poles (amphitely), in contrast with associations of sisters with only one pole (syntely) as is usually found during the first meiotic division. In several cases, prior to anaphase onset, maloriented bivalents appeared unusually tilted with respect to the spindle axis, and during anaphase they gave rise to laggard half-bivalents that did not segregate during anaphase along with half-bivalents having proper syntelic orientation. The results parallel previous findings obtained during cold recovery, and the properties of the drugs used here suggest that their action on microtubules, although reversible, induces malorientation during recovery from meiotic arrest.

References

Mar 22, 1976·Biochemical and Biophysical Research Communications·J HoebekeM De Brabander
May 1, 1970·The Journal of Cell Biology·J Aronson, S Inoué

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Citations

Feb 23, 2007·Molecular Biology of the Cell·Marie A JanickeJames R LaFountain
Apr 30, 2003·The Journal of Cell Biology·Alison L PidouxRobin C Allshire
Jun 14, 2008·Biochimica Et Biophysica Acta·Daniela Cimini
Jan 1, 1986·Cell Motility and the Cytoskeleton·M A Janicke, J R LaFountain
May 16, 2003·Current Biology : CB·Alison Pidoux, Robin Allshire
Feb 11, 2011·Trends in Cell Biology·Juraj GreganDaniela Cimini

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