MALS-3 regulates polarity and early neurogenesis in the developing cerebral cortex.

Development
Karpagam SrinivasanSusan K McConnell

Abstract

Apicobasal polarity plays an important role in regulating asymmetric cell divisions by neural progenitor cells (NPCs) in invertebrates, but the role of polarity in mammalian NPCs is poorly understood. Here, we characterize the function of the PDZ domain protein MALS-3 in the developing cerebral cortex. We find that MALS-3 is localized to the apical domain of NPCs. Mice lacking all three MALS genes fail to localize the polarity proteins PATJ and PALS1 apically in NPCs, whereas the formation and maintenance of adherens junctions appears normal. In the absence of MALS proteins, early NPCs progressed more slowly through the cell cycle, and their daughter cells were more likely to exit the cell cycle and differentiate into neurons. Interestingly, these effects were transient; NPCs recovered normal cell cycle properties during late neurogenesis. Experiments in which MALS-3 was targeted to the entire membrane resulted in a breakdown of apicobasal polarity, loss of adherens junctions, and a slowing of the cell cycle. Our results suggest that MALS-3 plays a role in maintaining apicobasal polarity and is required for normal neurogenesis in the developing cortex.

References

Jul 24, 1998·Molecular and Cellular Neurosciences·A ChennS K McConnell
Feb 7, 2001·The Journal of Biological Chemistry·E KamberovB Margolis
Jan 25, 2003·Nature Cell Biology·Roger Albertson, Chris Q Doe
Feb 5, 2003·The Journal of Cell Biology·Maria S BaldaKarl Matter
Aug 2, 2003·American Journal of Physiology. Renal Physiology·Michael H Roh, Ben Margolis
Aug 13, 2003·The EMBO Journal·Takeshi KawauchiMikio Hoshino
Nov 19, 2003·Mechanisms of Development·Andreas Wodarz, Wieland B Huttner
Jan 14, 2004·Molecular Biology of the Cell·Samuel W StraightBen Margolis
Mar 24, 2004·Genes & Development·Olga KlezovitchValeri Vasioukhin
Aug 5, 2004·The Journal of Biological Chemistry·Lin Gao, Ian G Macara
Oct 21, 2004·American Journal of Physiology. Renal Physiology·Olav OlsenPaul A Welling
Mar 2, 2005·The Journal of Cell Biology·Kunyoo ShinBen Margolis
Apr 22, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Dirk JunghansRolf Kemler
Jul 15, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Federico CalegariWieland B Huttner
Sep 24, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Julie A Siegenthaler, Michael W Miller
Sep 28, 2005·The Journal of Cell Biology·Olav OlsenDavid S Bredt
Oct 26, 2005·Current Opinion in Cell Biology·Wieland B Huttner, Yoichi Kosodo
Nov 11, 2005·Journal of Cell Science·Ben Margolis, Jean-Paul Borg
Nov 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bin ChenSusan K McConnell
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Magdalena Götz, Wieland B Huttner
Mar 1, 2006·Current Opinion in Cell Biology·Olav OlsenDavid S Bredt
Jun 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jennifer L FishWieland B Huttner
Aug 8, 2006·Nature Neuroscience·Silvia CappelloMagdalena Götz
Oct 3, 2006·The Journal of Biological Chemistry·Samuel W StraightBen Margolis
Dec 22, 2006·Molecular Biology of the Cell·Qian WangBen Margolis
Apr 10, 2007·Methods in Molecular Biology·Roger Vogelmann, W James Nelson

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Citations

Nov 22, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jinxiu ZhangJiafu Long
Apr 22, 2009·Development, Growth & Differentiation·Yoichi Kosodo, Wieland B Huttner
Mar 22, 2014·Nature Reviews. Neuroscience·Tao Sun, Robert F Hevner
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Maria K Lehtinen, Christopher A Walsh
Jun 10, 2009·The Journal of Cell Biology·Takashi IshiuchiTakuji Tanoue
Jan 24, 2016·Neuroscience Research·Jacobus J DudokJan Wijnholds
Nov 3, 2010·Current Opinion in Neurobiology·Simone A Fietz, Wieland B Huttner
Aug 26, 2014·Frontiers in Cellular Neuroscience·Marie-Theres SchmidMagdalena Götz
Aug 22, 2014·Cell and Tissue Research·Sophie LaguesseLaurent Nguyen
Aug 16, 2019·Genes, Brain, and Behavior·Tingting ZhangJiangang Gao
Feb 11, 2021·The FEBS Journal·Barry J Thompson

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