Malt sprout extract medium for cultivation of Lactobacillus plantarum protective cultures

Letters in Applied Microbiology
A LaitilaI Virkajärvi

Abstract

The aim was to develop a cheap cereal-based alternative medium for the large-scale production of biopreservative Lactobacillus plantarum VTT E-79098. We examined the effect of growth medium and pH control on the cell yield of Lact. plantarum E-79098 and the antimicrobial activity of the cell-free extracts. Fermentations using a novel Malt Sprout Extract Medium (MSE) were performed with different pH regimes. The antimicrobial activity of the cell-free extracts against Pantoea agglomerans VTT E-90396 and Fusarium avenaceum VTT D-80147 was assessed with automated turbidometry. When compared with MRS, the MSE medium cultures produced equal growth yields of Lact. plantarum VTT E-79098 and enhanced antimicrobial potential against the Gram-negative bacterium P. agglomerans and a Fusarium fungus. The MSE medium can be used as a low-cost alternative to MRS for producing high cell yields and good antimicrobial activity of Lact. plantarum.

References

Jan 1, 1995·International Journal of Food Microbiology·W H HolzapfelU Schillinger
Apr 13, 1999·Journal of Applied Microbiology·M L Niku-PaavolaA Haikara
Jul 16, 2002·Journal of Food Protection·J StilesL B Bullerman
Nov 1, 1995·Journal of Food Protection·Hassan Gourama, Lloyd B Bullerman

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Citations

Nov 26, 2009·Journal of Industrial Microbiology & Biotechnology·Graziela Brusch BrinquesMarco Antônio Záchia Ayub
Jul 28, 2016·International Journal of Food Sciences and Nutrition·Chaline Caren CoghettoMarco Antônio Záchia Ayub
Dec 1, 2017·Journal of Food Science and Technology·Luján CejasAndrea Gómez-Zavaglia

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