Mammalian BiP controls posttranslational ER translocation of the hepatitis B virus large envelope protein

FEBS Letters
Karin AweReinhild Prange

Abstract

The hepatitis B virus L protein forms a dual topology in the endoplasmic reticulum (ER) via a process involving cotranslational membrane integration and subsequent posttranslational translocation of its preS subdomain. Here, we show that preS posttranslocation depends on the action of the ER chaperone BiP. To modulate the in vivo BiP activity, we designed an approach based on overexpressing its positive and negative regulators, ER-localized DnaJ-domain containing protein 4 (ERdj4) and BiP-associated protein (BAP), respectively. The feasibility of this approach was confirmed by demonstrating that BAP, but not ERdj4, destabilizes the L/BiP complex. Overexpressing BAP or ERdj4 inhibits preS posttranslocation as does the reduction of ATP levels. These results hint to a new role of BiP in guiding posttranslational polypeptide import into the mammalian ER.

References

Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·J L BrodskyR Schekman
Jan 25, 2002·The Journal of Cell Biology·Nora G Haigh, Arthur E Johnson
Oct 3, 2002·The Journal of Biological Chemistry·Kyung Tae ChungLinda M Hendershot
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Carsten Lambert, Reinhild Prange
Dec 25, 2003·Journal of Virology·Christopher B BuckJohn T Schiller
Oct 31, 2006·Trends in Molecular Medicine·Richard ZimmermannBernd Wullich
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Gunnar von Heijne
Jan 9, 2007·World Journal of Gastroenterology : WJG·Dieter Glebe, Stephan Urban
Jan 9, 2007·World Journal of Gastroenterology : WJG·Volker Bruss
May 1, 2007·FEBS Letters·Danuta TomkiewiczArnold J M Driessen

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Citations

Sep 12, 2012·Medical Microbiology and Immunology·Reinhild Prange
May 13, 2010·Archives of Virology·Allen XiaoHonglin Luo
Oct 20, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·John A PapalasMaria A Selim
Aug 13, 2010·Molecular & Cellular Proteomics : MCP·Kevin B SpurgersSina Bavari
Aug 13, 2009·Molecular & Cellular Proteomics : MCP·Yan MaMing-liang He
Dec 6, 2011·PLoS Neglected Tropical Diseases·Mohammed Abdelfatah AlhootShamala Devi Sekaran
Mar 30, 2012·PloS One·Mohammed Abdelfatah AlhootShamala Devi Sekaran
Oct 21, 2009·World Journal of Gastroenterology : WJG·Xiao ZhuHsiang-Fu Kung
Jul 16, 2013·Nature Chemical Biology·Maike C JürgensNeil Ferguson
Dec 23, 2009·Seminars in Cell & Developmental Biology·Joel H OteroLinda M Hendershot
Jan 21, 2016·Oxidative Medicine and Cellular Longevity·Tommaso MelloAndrea Galli
Mar 8, 2014·Journal of Medical Virology·Sayeh EzzikouriKyoko Tsukiyama-Kohara
Dec 15, 2010·Journal of Hepatology·Harmeet Malhi, Randal J Kaufman
Apr 20, 2016·Expert Review of Gastroenterology & Hepatology·Fa-Ling WuMing-Hua Zheng
Sep 1, 2009·Viruses·Stephanie Schädler, Eberhard Hildt
Mar 13, 2019·Cold Spring Harbor Perspectives in Biology·Ranen Aviner, Judith Frydman
May 8, 2020·Histochemistry and Cell Biology·Lea Daverkausen-FischerFelicitas Pröls
Feb 16, 2016·World Journal of Gastroenterology : WJG·Yong-Wei LiJiong-Shan Zhang
Dec 1, 2017·Biological & Pharmaceutical Bulletin·Ying YangYue Gao
Apr 3, 2019·The Protein Journal·William DowhanMikhail Bogdanov
Jan 26, 2021·Microbiology and Immunology·Yadarat SuwanmaneeKeiji Ueda
Aug 7, 2021·Cell Death Discovery·Lea Daverkausen-Fischer, Felicitas Pröls
Jul 6, 2010·Biochimica Et Biophysica Acta·Richard ZimmermannVolkhard Helms

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