Mammalian cell-entry proteins encoded by the mce3 operon of Mycobacterium tuberculosis are expressed during natural infection in humans

Scandinavian Journal of Immunology
S AhmadR Al-Attiyah

Abstract

The mammalian cell-entry (mce)3 operon is one of four homologous mce operons on Mycobacterium tuberculosis genome that encodes six putative invasin/ adhesin-like proteins (Mce3A-F) possibly involved in the entry and survival of this bacterium inside macrophages. To study the in vivo expression of the mce3 operon-encoded proteins during natural human infection, the genes encoding Mce3A-F were cloned and expressed in Escherichia coli as fusion proteins with glutathione-S-transferase (GST) at the N-terminal and a x6 histidine (His) tag at the C-terminal end. The recombinant proteins appeared as major cellular proteins in SDS-PAGE gels and reacted with anti-GST and antipenta-His antibodies at the expected molecular mass of 70, 61, 68, 71, 66 and 72 [corrected] kDa for GST-Mce3A, GST-Mce3B, GST-Mce3C, GST-Mce3D, GST-Mce3E and GST-Mce3F, respectively. In Western immunoblots, all the six fusion proteins, particularly GST-Mce3A, GST-Mce3C, GST-Mce3D and GST-Mce3E, reacted with antibodies in combined human serum from 11 tuberculosis (TB) patients. Pure Mce3A, Mce3D and Mce3E could be isolated by specific proteolytic cleavage by thrombin protease of the respective purified fusion protein followed by preparative SDS-PAGE. The pure Mce3A, ...Continue Reading

References

Jun 8, 1999·Annual Review of Immunology·A Aderem, D M Underhill
Nov 11, 1999·International Journal of Systematic Bacteriology·S AhmadR K Bhatnagar
Mar 1, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·F TekaiaS T Cole
Apr 12, 2000·International Journal of Systematic and Evolutionary Microbiology·S AhmadB K Patel
Dec 19, 2001·Infection and Immunity·Maddalena PanigadaFabio Grassi
Jun 8, 2002·FEMS Immunology and Medical Microbiology·Yoseph HaileHarald G Wiker
Oct 9, 2002·Microbiology·María P SantangeloFabiana Bigi
Dec 11, 2002·Scandinavian Journal of Immunology·M HarboeH G Wiker
Jun 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Thierry GarnierR Glyn Hewinson
Sep 19, 2003·Lancet·Thomas R FriedenChristopher Dye
Sep 30, 2003·Nature Immunology·Suzanne M Hingley-WilsonWilliam R Jacobs
Nov 1, 2003·The Journal of Infectious Diseases·Peter SeilerPeter Aichele

❮ Previous
Next ❯

Citations

Dec 14, 2011·Applied Microbiology and Biotechnology·Sasikala SivagnanamRajamanickam Chellam
Aug 5, 2011·Scandinavian Journal of Immunology·S N M HanifA S Mustafa
May 15, 2010·Microbiology·Nancy MahMiguel A Andrade-Navarro
Apr 1, 2011·Veterinary Immunology and Immunopathology·Richard J WhittingtonAuriol C Purdie
Jan 20, 2015·Nature Genetics·Matthias MerkerThierry Wirth
Jan 15, 2011·Clinical & Developmental Immunology·Suhail Ahmad
Jul 30, 2016·Microbiology and Immunology·Maria Raquel CosateAna Lucia T O Nascimento
Jan 19, 2018·Journal of Leukocyte Biology·Michael D StutzMarc Pellegrini
Sep 6, 2008·Nature Protocols·Darren J Korbie, John S Mattick
Aug 5, 2017·International Journal of Mycobacteriology·Shumaila N M Hanif, A S Mustafa
Jan 19, 2008·Journal of Medical Microbiology·Ryan H SenaratneLee W Riley
Mar 18, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jie LiCui Hua Liu
Jan 25, 2011·Molecular and Cellular Biochemistry·Fan Zhang, Jian-Ping Xie

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.