Mammalian cell toxicity and candidacidal mechanism of Arg- or Lys-containing Trp-rich model antimicrobial peptides and their d-enantiomeric peptides

Peptides
Yong Hai NanSong Yub Shin

Abstract

In this study, we investigated the mammalian cell toxicity and candidacidal mechanism of Arg- or Lys-containing Trp-rich model antimicrobial peptides (K(6)L(2)W(3) and R(6)L(2)W(3)) and their d-enantiomeric peptides (K(6)L(2)W(3)-d and R(6)L(2)W(3)-d). Arg-containing peptides were more toxic to human erythrocytes and mammalian cells as compared to Lys-containing peptides. Arg-containing peptides is slightly more hydrophobic than Lys-containing counterparts, as judged from their reverse phase-high performance liquid chromatography (RP-HPLC) retention time. These results suggested that a little difference in hydrophobicity of these peptides affect their hemolytic activity and mammalian cell toxicity. Interestingly, K(6)L(2)W(3) and K(6)L(2)W(3)-d almost similar mammalian cell cytotoxicity, whereas R(6)L(2)W(3)-d showed much higher cytotoxicity as compared to R(6)L(2)W(3). A low ability to facilitate fluorescent marker escape from Candida albicans membrane-mimicking vesicles suggested that the major target site of Lys-containing peptides may be not the cell membrane but the cytoplasm of C. albicans. Confocal laser-scanning microscopy revealed that FITC-labeled Lys-containing peptides penetrated the cell wall and cell membrane and ...Continue Reading

References

Jan 1, 1987·Critical Reviews in Microbiology·F C Odds
Jan 1, 1995·Annual Review of Immunology·H G Boman
May 1, 1995·Journal of Dental Research·R D CannonB C Monk
Feb 8, 1997·Lancet·R E Hancock
Oct 21, 1998·Biochemistry·W M YauS H White
Dec 11, 1999·Biochimica Et Biophysica Acta·R M Epand, H J Vogel
Sep 6, 2000·Trends in Biochemical Sciences·J A Killian, G von Heijne
Jan 25, 2002·Nature·Michael Zasloff
May 7, 2002·The Journal of Biological Chemistry·Hongxia Zhao, Paavo K J Kinnunen
Nov 21, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David J SchibliRichard M Epand
Dec 16, 2004·International Review of Cytology·Rajendra Prasad, Khyati Kapoor
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Mónica Viejo-DíazJosé F Fierro
Aug 2, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Laszlo Otvos
May 25, 2006·The Journal of Biological Chemistry·Xuewei S LiMira Edgerton
Jun 8, 2006·Biochimica Et Biophysica Acta·David I ChanHans J Vogel
Oct 7, 2006·The Journal of Antimicrobial Chemotherapy·Monica BenincasaRenato Gennaro
Oct 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Arik MakovitzkiYechiel Shai

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Citations

Dec 4, 2012·International Journal of Molecular Sciences·Ramamourthy GopalYoonkyung Park
Oct 31, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Yong Hai NanSong Yub Shin
Oct 1, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Yong Hai NanSong Yub Shin
Aug 29, 2013·Journal of the Science of Food and Agriculture·Muhammad Aqeel AshrafMuhammad Abdur Rehman
Aug 28, 2015·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ganesan RajasekaranSong Yub Shin
Jul 24, 2015·Biomolecular Concepts·Małgorzata Cytryńska, Agnieszka Zdybicka-Barabas
Sep 6, 2014·Molecular Pharmaceutics·Karsten RapschMarkus von Nickisch-Rosenegk
Mar 1, 2012·Comprehensive Reviews in Food Science and Food Safety·Paula Judith Perez EspitiaEber Antonio Alves Medeiros

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