Mammalian cells use the autophagy process to restrict avian influenza virus replication.

Cell Reports
Siwen LiuHonglin Chen

Abstract

Host adaptive mutations in the influenza A virus (IAV) PB2 protein are critical for human infection, but their molecular action is not well understood. We observe that when IAV containing avian PB2 infects mammalian cells, viral ribonucleoprotein (vRNP) aggregates that localize to the microtubule-organizing center (MTOC) are formed. These vRNP aggregates resemble LC3B-associated autophagosome structures, with aggresome-like properties, in that they cause the re-distribution of vimentin. However, electron microscopy reveals that these aggregates represent an accumulation of autophagic vacuoles. Compared to mammalian-PB2 virus, avian-PB2 virus induces higher autophagic flux in infected cells, indicating an increased rate of autophagosomes containing avian vRNPs fusing with lysosomes. We found that p62 is essential for the formation of vRNP aggregates and that the Raptor-interacting region of p62 is required for interaction with vRNPs through the PB2 polymerase subunit. Selective autophagic sequestration during late-stage virus replication is thus an additional strategy for host restriction of avian-PB2 IAV.

References

Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·S R ShihR M Krug
Dec 29, 1998·The Journal of Cell Biology·J A JohnstonR R Kopito
Jun 1, 2004·Journal of Cell Science·Yukiko KabeyaTamotsu Yoshimori
Dec 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·G GabrielJ Stech
Feb 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·H ChenY Guan
Nov 1, 2006·Emerging Infectious Diseases·Guang-Wu ChenShin-Ru Shih
Dec 7, 2006·PLoS Biology·María A RujanoHarm H Kampinga
Jun 21, 2007·The Journal of Biological Chemistry·Serhiy PankivTerje Johansen
Oct 2, 2007·Microbes and Infection·Fumitaka MomoseYuko Morikawa
Apr 5, 2008·Cell Structure and Function·Shunsuke KimuraTamotsu Yoshimori
Jun 12, 2008·International Review of Cell and Molecular Biology·Eeva-Liisa Eskelinen
Sep 13, 2008·Annual Review of Microbiology·Nadia NaffakhSylvie van der Werf
Oct 20, 2009·Cell Host & Microbe·Monique GannagéChristian Münz
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andrew Mehle, Jennifer A Doudna
Feb 11, 2010·Cell·Noboru MizushimaBeth Levine
Jun 15, 2010·Cell Host & Microbe·Jeffery K Taubenberger, John C Kash
Apr 29, 2011·Journal of Virology·Amie J EisfeldYoshihiro Kawaoka
Oct 11, 2011·Molecular Cell·Angeles DuranMaria T Diaz-Meco
May 3, 2012·Nature Communications·Benjamin MänzMartin Schwemmle
May 24, 2012·Autophagy·Dandan Chen, Qing Zhong
Apr 13, 2013·The New England Journal of Medicine·Rongbao GaoYuelong Shu
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Mar 4, 2014·The Journal of General Virology·Anna V CauldwellWendy S Barclay
Mar 29, 2014·The EMBO Journal·Miranda de Graaf, Ron A M Fouchier
May 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Mikołaj OgrodnikDaniel Kaganovich
Aug 15, 2014·Nature Reviews. Microbiology·Annabel GuichardEthan Bier
Sep 17, 2014·Nature Communications·Edward C HutchinsonErvin Fodor
Feb 1, 2014·Emerging Microbes & Infections·Eefje Ja Schrauwen, Ron Am Fouchier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms