Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates

The Journal of Cell Biology
Naomi BishopP Woodman

Abstract

There is increasing evidence that ubiquitination of receptors provides an important endosomal sorting signal. Here we report that mammalian class E vacuolar protein-sorting (vps) proteins recognize ubiquitin. Both tumor susceptibility gene 101 (TSG101)/human VPS (hVPS)28 and hepatocyte growth factor receptor substrate (Hrs) cytosolic complexes bind ubiquitin-agarose. TSG101 and hVPS28 are localized to endosomes that contain internalized EGF receptor and label strongly for ubiquitinated proteins. Microinjection of anti-hVPS28 specifically retards EGF degradation and leads to endosomal accumulation of ubiquitin-protein conjugates. Likewise, depletion of TSG101 impairs EGF trafficking and causes dramatic relocalization of ubiquitin to endocytic compartments. Similar defects are found in cells overexpressing Hrs, further emphasizing the links between class E protein function, receptor trafficking, and endosomal ubiquitination.

References

Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·U SchubertJ W Yewdell
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·B StrackH G Gottlinger
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·A PatnaikJ W Wills
Jan 13, 2001·The Journal of Biological Chemistry·N Bishop, P Woodman
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L VerPlankC A Carter

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Citations

Sep 25, 2003·Cell·Uta K von SchwedlerWesley I Sundquist
Oct 18, 2002·Current Opinion in Cell Biology·Hugh R B Pelham
Aug 2, 2003·Current Opinion in Cell Biology·Jean Gruenberg
Aug 2, 2003·Current Opinion in Cell Biology·Camilla RaiborgHarald Stenmark
Aug 21, 2002·Trends in Cell Biology·F Gisou van der Goot, Jean Gruenberg
Dec 24, 2002·Trends in Cell Biology·Owen PornillosWesley I Sundquist
Feb 25, 2003·Trends in Microbiology·Eric O Freed
Apr 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Aurelie DoyottePhilip Woodman
May 21, 2010·The Journal of Biological Chemistry·Eugene L KangGiuseppina Tesco
Sep 3, 2002·The Journal of Biological Chemistry·Andrea KremplerKay-Uwe Wagner
May 30, 2008·The Journal of Biological Chemistry·Jihui RenRobert C Piper
Apr 11, 2003·Molecular Biology of the Cell·Dean E HammondMichael J Clague
Oct 8, 2004·Molecular Biology of the Cell·Bin ZhengMarilyn G Farquhar
Oct 7, 2005·Molecular Biology of the Cell·Zhe-Yu ChenFrancis S Lee
May 19, 2006·Molecular Biology of the Cell·M Razi, C E Futter
Jul 14, 2006·Molecular Biology of the Cell·Tor Erik RustenHarald Stenmark
Mar 3, 2007·Molecular Biology of the Cell·Joanna C BakowskaCraig Blackstone
May 4, 2007·Molecular Biology of the Cell·Ivana De DomenicoJerry Kaplan
Dec 21, 2007·Journal of Virology·Benjamin G LuttgeEric O Freed
Mar 29, 2003·Journal of Virology·Juan Martin-SerranoPaul D Bieniasz
Dec 25, 2003·Journal of Virology·Miranda Shehu-XhilagaEric O Freed
Oct 28, 2004·Journal of Virology·Rajeshwari R Valiathan, Marilyn D Resh
Apr 25, 2007·Molecular and Cellular Biology·Sandra NiendorfKlaus-Peter Knobeloch
May 4, 2004·Molecular and Cellular Biology·Otilia V VieiraSergio Grinstein
Mar 26, 2003·Annual Review of Biochemistry·Juan S Bonifacino, Linton M Traub
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·James H Hurley, Scott D Emr
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Linda Hicke, Rebecca Dunn
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Eiji Morita, Wesley I Sundquist

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Methods Mentioned

BETA
ubiquitination
deubiquitination
fluorescence microscopy
FCS

Software Mentioned

Metamorph

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