Mammalian embryo comparison identifies novel pluripotency genes associated with the naïve or primed state.

Biology Open
Andreia S BernardoAndras Dinnyes

Abstract

During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, 'naïve' or 'primed', depends on the embryonic stage and derivation conditions used. Here we analyse the temporal gene expression patterns of mouse, cattle and porcine embryos at stages that harbour different types of pluripotent cells. We document conserved and divergent traits in gene expression, and identify predictor genes shared across the species that are associated with pluripotent states in vivo and in vitro Amongst these are the pluripotency-linked genes Klf4 and Lin28b The novel genes discovered include naïve- (Spic, Scpep1 and Gjb5) and primed-associated (Sema6a and Jakmip2) genes as well as naïve to primed transition genes (Dusp6 and Trip6). Both Gjb5 and Dusp6 play a role in pluripotency since their knockdown results in differentiation and downregulation of key pluripotency genes. Our interspecies comparison revealed new insights of pluripotency, pluripotent stem cell identity and a new molecular criterion for distinguishing between pluripotent states in various species, including human.

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Citations

Feb 16, 2019·Epigenetics & Chromatin·René A M DirksHendrik Marks
Feb 20, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jinghui GaoAmy L Ryan
Jul 25, 2019·Frontiers in Endocrinology·Teresa ChioccarelliRosanna Chianese
Nov 7, 2019·Genetics·Joel Rothman, Sophie Jarriault
Dec 10, 2020·BMC Veterinary Research·Rachel A ScarfoneThomas G Koch
Mar 6, 2021·Veterinary Research·Bertrand Pain
Jun 23, 2021·Stem Cell Reviews and Reports·Mohammed AbdulhasanDaniel A Rappolee
Aug 14, 2021·Frontiers in Cell and Developmental Biology·Vera A van der Weijden, Aydan Bulut-Karslioglu

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Datasets Mentioned

BETA
GSE53387

Methods Mentioned

BETA
RNA-seq
PCR
Flow cytometry

Software Mentioned

SMARTer
FlowJo
UCSC browser
BedGraph

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