PMID: 9653110Jul 8, 1998Paper

Mammalian Golgi apparatus UDP-N-acetylglucosamine transporter: molecular cloning by phenotypic correction of a yeast mutant

Proceedings of the National Academy of Sciences of the United States of America
Eduardo GuillenC B Hirschberg

Abstract

Transporters in the Golgi apparatus membrane translocate nucleotide sugars from the cytosol into the Golgi lumen before these can be substrates for the glycosylation of proteins, lipids, and proteoglycans. We have cloned the mammalian Golgi membrane transporter for uridine diphosphate-N-acetylglucosamine by phenotypic correction with cDNA from MDCK cells of a recently characterized Kluyveromyces lactis mutant deficient in Golgi transport of the above nucleotide sugar. Phenotypically corrected transformants were separated from mutants in a fluorescent-activated cell sorter after labeling of K. lactis cells with fluorescein isothiocyanate (FITC) conjugated to Griffonia simplicifolia II lectin, which binds terminal N-acetylglucosamine. A 2-kb DNA fragment was found to restore the wild-type cell lectin binding phenotype, which reverted to the mutant one upon loss of the plasmid. The DNA fragment contained an ORF encoding a hydrophobic, multitransmembrane spanning protein of 326 aa that had only 22% amino acid sequence identity with the corresponding transporter from K. lactis but showed 53% amino acid sequence identity to the mammalian UDP-galactose transporters and 40% to the CMP-sialic acid transporter. Golgi vesicles from the tr...Continue Reading

References

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Citations

Sep 1, 2001·Biochimie·R Gerardy-SchahnH Bakker
Oct 24, 2000·Current Opinion in Structural Biology·P M Berninsone, C B Hirschberg
Mar 10, 2007·The Journal of Biological Chemistry·Althea A CapulStephen M Beverley
Jun 5, 2002·Annual Review of Biochemistry·Jeffrey D Esko, Scott B Selleck
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Carolina E CaffaroPatricia M Berninsone
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·P BerninsoneC B Hirschberg
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Byung-Kwon ChoiTillman U Gerngross
Mar 20, 2013·Molecular Aspects of Medicine·Zhiwei Song
Nov 22, 2015·BMC Microbiology·Carlos Gustavo BaptistaAugusto Savio Peixoto Ramos
Jan 26, 2006·Biochimica Et Biophysica Acta·Mariusz Olczak, Eduardo Guillen
Apr 16, 2013·Biochemical and Biophysical Research Communications·Mariusz OlczakTeresa Olczak
May 1, 2007·Biochimica Et Biophysica Acta·Miklós CsalaGábor Bánhegyi
Jun 5, 2016·Journal of Molecular Biology·Ariel OrellanaAdrian Moreno
Oct 16, 2016·Biotechnology Letters·Karin Förster-FrommeChristoph Albermann
Mar 23, 2017·The Journal of Clinical Endocrinology and Metabolism·Willy MorelleFrançois Foulquier
Jul 20, 2005·The Journal of Biological Chemistry·Luis M BredestonCarlos B Hirschberg
Feb 6, 1999·The Journal of Biological Chemistry·L PuglielliC B Hirschberg
Dec 10, 1999·The Journal of Biological Chemistry·L Puglielli, C B Hirschberg
Mar 20, 1999·The Journal of Biological Chemistry·M EckhardtR Gerardy-Schahn
Apr 30, 2003·The Journal of Biological Chemistry·Shin KamiyamaShoko Nishihara
Jul 27, 2007·The Journal of Biological Chemistry·Carolina E CaffaroPatricia M Berninsone
Aug 19, 2018·The Journal of Biological Chemistry·Carlos B Hirschberg
Jun 15, 2013·The Journal of Biological Chemistry·Dorota Maszczak-SeneczkoMariusz Olczak
Nov 16, 2004·The Journal of Biological Chemistry·Hiroaki SegawaSalvatore J Turco

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