Mammalian histone deacetylase 1 protein is posttranslationally modified by phosphorylation

Biochemical and Biophysical Research Communications
R L CaiD Cohen

Abstract

HDAC1, a member of the histone deacetylase family, is involved in transcription regulation through the modification of chromatin structure. Several studies also implicated HDAC1 in tumorigenesis. Much attention has been concentrated on protein-protein interactions involving HDAC1 and the possibility that posttranslational modifications may occur in mammalian HDAC1 proteins has not been carefully and systematically investigated. In this study, we utilized in vivo labeling assays to demonstrate that both human and murine HDAC1 proteins are phosphorylated in cells. Assays using HDAC1 deletion mutants indicated that phosphorylation occurs in its C-terminal domain. cAMP-dependent kinase and casein kinase II, but not protein kinase C, cdc2, or MAP kinase, could phosphorylate HDAC1 in vitro, although HDAC1 contains several protein kinase C consensus sites. We also found that phosphorylation did not influence HDAC1 enzymatic activity using a human histone H4 N-terminal peptide as the substrate. Interestingly, HDAC1-FLAG fusion protein immunoprecipitated from transfected cells was found to be in association with a kinase activity, providing an in vitro assay for further studies of this posttranslational modification.

References

Jun 28, 1996·The Journal of Biological Chemistry·A A CarmenM Grunstein
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·W M YangE Seto
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S E RundlettM Grunstein
Sep 1, 1997·Molecular and Cellular Biology·S BartlC Seiser
Feb 12, 1998·Biochemical and Biophysical Research Communications·Y SowaT Sakai
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·S EmilianiE Verdin
Jun 10, 1998·Biochemical and Biophysical Research Communications·L CuissetM Delpech
Oct 1, 1998·Biochemical and Biophysical Research Communications·R L Cai
Apr 17, 1999·The Journal of Biological Chemistry·W FischleE Verdin
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·C M GrozingerS L Schreiber
Sep 24, 1999·Biochemical and Biophysical Research Communications·U MahlknechtE Verdin
Jun 28, 2000·Proceedings of the National Academy of Sciences of the United States of America·C M Grozinger, S L Schreiber
Aug 25, 2000·Molecular and Cellular Biology·A H WangX J Yang

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Citations

Mar 25, 2008·Cellular and Molecular Life Sciences : CMLS·D P DowlingD W Christianson
Mar 13, 2009·The Journal of Biological Chemistry·Su-Jin KimChristopher H S McIntosh
Jan 1, 2004·Molecular and Cellular Biology·Heehyoung LeeEdward Seto
Jan 5, 2011·Journal of Biomedicine & Biotechnology·Chiara V Segré, Susanna Chiocca
Oct 7, 2006·BMC Biochemistry·Nayana KamathMary Kay H Pflum
Mar 8, 2013·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Michael J Morris, Lisa M Monteggia
Jan 12, 2008·Advances in Enzyme Regulation·James R DavieXuemei Wang
Oct 16, 2007·International Journal of Cancer. Journal International Du Cancer·Sireerat PluemsampantIkuo Morita
Feb 12, 2009·Biology of the Cell·André BrandlOliver H Krämer
Jul 24, 2007·Biochemical and Biophysical Research Communications·Paulina Karwowska-DesaulniersMary Kay H Pflum
Dec 3, 2016·Molecular Biology of the Cell·Katarzyna Chmielarska MasoumiRamin Massoumi
Jan 30, 2004·International Journal of Cancer. Journal International Du Cancer·Hanako KobayashiSharon E Fleming
Feb 28, 2007·Cell Research·Paola GallinariChristian Steinkühler
Sep 8, 2004·Journal of Cellular Biochemistry·Nilanjan Sengupta, Edward Seto
Dec 14, 2002·Genesis : the Journal of Genetics and Development·Marija ZeremskiDalia Cohen
Jun 26, 2002·The Journal of Biological Chemistry·Shih-Chang Tsai, Edward Seto
Oct 2, 2002·The Journal of Biological Chemistry·Hirotaka SakaiFuyuki Ishikawa
Jan 18, 2007·Molecular Endocrinology·Sayura Aoyagi, Trevor K Archer
Oct 17, 2001·The Journal of Biological Chemistry·M K PflumS L Schreiber
Nov 20, 2016·Chembiochem : a European Journal of Chemical Biology·D Maheeka Embogama, Mary Kay H Pflum
Aug 15, 2002·The Journal of Biological Chemistry·Jian-Min SunJames R Davie
Dec 27, 2005·The Journal of Biological Chemistry·Ming-Chuan HsuWen-Chun Hung
Mar 29, 2002·The Journal of Biological Chemistry·Scott C GalasinskiNatalie G Ahn
Nov 24, 2012·Biopolymers·Noah A WolfsonCarol A Fierke
Oct 12, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Di FengBetsy J Barnes
Jul 20, 2020·Cellular and Molecular Life Sciences : CMLS·Sonali Bahl, Edward Seto
Mar 14, 2013·International Journal of Oncology·Martine Müller KlosterSoheil Naderi
Nov 15, 2020·International Journal of Molecular Sciences·Min Young KimYi Qiu
Sep 30, 2006·Cellular Signalling·Changyue GuoJames M Larner

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